A conjugate of an oligosaccharide fragment of tumor-associated ganglioside antigen with hemocyanin is a prototype antitumor vaccine
| Autor: | V. L. Lvov, Nikolay E. Nifant'ev, R. N. Stepanenko, R. Ya. Vlasenko, Elena A. Khatuntseva, Yu. E. Tsvetkov, R. V. Petrov |
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| Rok vydání: | 2007 |
| Předmět: |
chemical and pharmacologic phenomena
Enzyme-Linked Immunosorbent Assay Biology Cancer Vaccines General Biochemistry Genetics and Molecular Biology Mice Immune system Antigen Antigens Neoplasm Animals G(M3) Ganglioside Vaccines Conjugate General Immunology and Microbiology Immunogenicity General Medicine Virology Molecular biology Tumor antigen Mice Inbred C57BL Immunization Macrophage-1 antigen Hemocyanins biology.protein Antibody General Agricultural and Biological Sciences Conjugate |
| Zdroj: | Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections. 415 |
| ISSN: | 0012-4966 |
| Popis: | Specific stimulation of the immune system against tumor antigens, which may result in elimination of malignant cells, may be performed with the use of vaccines containing a specific native or synthetic tumorassociated antigen. To develop a prototype antitumor vaccine, we synthesized an oligosaccharide fragment of tumor-associated antigen, obtained its conjugate with the protein hemocyanin, and studied the immunogenicity of this conjugate. We developed a technique of immunization of experimental animals by the conjugate and obtained secondary immune response to the tumor antigen with a predominant generation of IgG antibodies (ABs). We showed that the ABs generated after immunization of animals with the use of conjugate reacted with the native tumor-associated antigen. The serum of animals immunized with the conjugate contained IgG1 and IgG3 ABs necessary for specific interaction of macrophages and natural killers with malignant cells; hence, the conjugate may be used as a prototype antitumor vaccine. |
| Databáze: | OpenAIRE |
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