Modified Renshen Yangrong decoction enhances angiogenesis in ischemic stroke through promotion of MicroRNA‐210 expression by regulating the HIF/VEGF/Notch signaling pathway
| Autor: | Xu-Liang Shi, Lin Jia, Cui-Huan Yan, Ya-Li Wang, Ce Liang, Teng Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Angiogenesis Renshen Yangrong decoction Notch signaling pathway Neurosciences. Biological psychiatry. Neuropsychiatry Brain Ischemia Behavioral Neuroscience angiogenesis HIF/VEGF/Notch In vivo Internal medicine medicine Animals Original Research Tube formation Ischemic stroke business.industry Cell growth Hypoxia (medical) Rats Stroke MicroRNAs Endocrinology miRNA‐210 Ephrin A3 medicine.symptom Endostatin business Drugs Chinese Herbal Signal Transduction RC321-571 |
| Zdroj: | Brain and Behavior, Vol 11, Iss 8, Pp n/a-n/a (2021) Brain and Behavior |
| ISSN: | 2162-3279 |
| Popis: | Objective This study aims to investigate the efficacy of modified Ginseng Yangrong decoction (GSYRD) promoting angiogenesis after ischemic stroke. Methods In an in vivo study, rats that survived surgery were allocated into four groups: the control group and model group were treated with normal saline, the GSYRD group was treated with 18.9 mg/kg of GSYRD daily, and the positive control group was treated with Tongxinluo (TXL) (1 g/kg/d). At the end of the seven‐day treatment, the area of cerebral infarction, the expression changes of miRNA‐210 and ephrin A3 were determined. In an in vitro study, HUVECs were divided into a normal control serum group (NC group), normal control serum OGD group (Oxygen Glucose Deprivation group) (OGD group), OGD + drug‐containing serum group (OGD+GSYRD group), and OGD + drug‐containing serum + ES group (Endostatin group) (OGD+GSYRD+ES group). The cells in all groups except the NC group were cultured in a sugar‐free DMEM medium under hypoxia for 48 h. Cell proliferation, angiogenic structure formation ability, the expression changes of miRNA‐210, ephrin A3, and the HIF/VEGF/Notch signaling pathway‐related molecules were determined. Results In vivo, GSYRD significantly reduced infarct size (p This study aims to investigate the efficacy of modified Ginseng Yangrong decoction (GSYRD) promoting angiogenesis after ischemic stroke. GSYRD promotes cerebral protection following angiogenesis and ischemic brain injury. The specific mechanism was activating the HIF/VEGF/Notch signaling pathway via miRNA‐210. |
| Databáze: | OpenAIRE |
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