Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK
| Autor: | Juin-Hong Cherng, Yi-Huei Huang, Cheng-Che Liu, Po-Da Hong, Hui-Wen Chuang, Kuan-Yi Yu, Shou-Ping Huang, Yao-Wen Hung, Wen-Zhi Lin, Ming-Kung Yeh |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
viruses Clinical Biochemistry Population BK polyomavirus JC virus 030230 surgery medicine.disease_cause Virus Article law.invention 03 medical and health sciences 0302 clinical medicine Plasmid law medicine education Polymerase chain reaction education.field_of_study lcsh:R5-920 Chemistry Oligonucleotide nucleic acid testing lateral flow immunoassay virus diseases Virology BK virus point-of-care testing 030104 developmental biology Nucleic acid lcsh:Medicine (General) |
| Zdroj: | Diagnostics, Vol 10, Iss 403, p 403 (2020) Diagnostics Volume 10 Issue 6 |
| ISSN: | 2075-4418 |
| Popis: | The BK virus (BKV) is an emerging pathogen in immunocompromised individuals and widespread in the human population. Polymerase chain reaction is a simple and highly sensitive method for detecting BKV, but it is time consuming and requires expensive instruments and expert judgment. The lateral flow assay, a rapid, low-cost, minimal-labor, and easy-to-use diagnostic method, was successfully applied for pathogen detection. In this study, we used oligonucleotide probes to develop a simple and rapid sandwich-type lateral flow immunoassay for detecting BKV DNA within 45 minutes. The detection limit for the synthetic single-stranded DNA was 5 nM. The specificity study showed no cross-reactivity with other polyomaviruses, such as JC virus and simian virus 40. For the Escherichia coli containing BKV plasmid cultured samples, the sensitivity was determined to be 107 copies/mL. The approach offers great potential for BKV detection of various target analytes in point-of-care settings. |
| Databáze: | OpenAIRE |
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