Erratum to: Attempts to improve human ovarian transplantation outcomes of needle immersed vitrification and slow-freezing by host and graft treatments
| Autor: | Raoul Orvieto, Galit Lerer-Serfaty, Noa Fisher, Michal Herman-Edelstein, Avi Ben-Haroush, Anat Stein, Roei Magen, Ronit Abir, Benjamin Fisch, Nivin Samara |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult medicine.medical_specialty Cell Survival Ovary Apoptosis Biology Cryopreservation Melatonin Andrology 03 medical and health sciences Mice 0302 clinical medicine Ovarian Follicle Pregnancy Freezing Genetics medicine Animals Humans Vitrification Embryo Implantation Ovarian follicle Incubation Genetics (clinical) Slow freezing 030219 obstetrics & reproductive medicine Obstetrics and Gynecology General Medicine Ovarian transplantation Surgery Platelet Endothelial Cell Adhesion Molecule-1 030104 developmental biology medicine.anatomical_structure Ki-67 Antigen Reproductive Medicine Female Erratum Developmental Biology medicine.drug |
| Popis: | To investigate if needle-immersed vitrification or slow-freezing yields better implantation results for human ovarian tissue and which method benefits more when combined with the "improvement protocol" of host melatonin treatment and graft incubation with biological glue + vitamin E + vascular endothelial growth factor-A.Human ovarian tissue was preserved by needle-immersed vitrification or slow-freezing and transplanted into immunodeficient mice, either untreated (groups A and C, respectively) or treated with the improvement protocol (groups B and D, respectively). Grafted and ungrafted slices were evaluated by follicle counts, apoptosis assay and immunohistochemistry for Ki67 and platelet endothelial cell adhesion molecule (PECAM).Follicle number in the recovered grafts was limited. The number of atretic follicles was significantly higher after vitrification with/without the improvement protocol and slow-freezing than that after slow-freezing + the improvement protocol. Stroma cell apoptosis was the lowest in the group D. PECAM staining showed a peripheral and diffuse pattern in the group D (mostly normal follicular morphology) and a diffuse pattern in all other groups (few follicles, mostly atretic), with significantly higher diffuse levels in the vitrification groups. Ki67 staining was identified in all normal follicles. Follicles did not survive transplantation in the vitrification groups.Ovarian sample preparation with slow-freezing + the improvement protocol appears to yield better implantation outcomes than needle-immersed vitrification with/without the improvement protocol. The real quality of frozen tissue can be assessed only after grafting and not after thawing/warming. |
| Databáze: | OpenAIRE |
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