Diterpenes inhibit IL-12 production by DC and enhance Th2 cells polarization
| Autor: | Akemi Umeyama, Shigenobu Arihara, Masao Takei |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Lipopolysaccharides
medicine.medical_treatment Cell Biophysics Immunoglobulins Biology Biochemistry Proinflammatory cytokine Th2 Cells Antigens CD Cell polarity medicine Humans Molecular Biology Secoferruginol Membrane Glycoproteins Effector Cell Polarity Dendritic Cells Cell Biology Interleukin-12 Phenotype Cell biology medicine.anatomical_structure Cytokine Immunology Interleukin 12 Diterpenes |
| Zdroj: | Biochemical and Biophysical Research Communications. 355:603-610 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2007.01.157 |
| Popis: | Sugiol and 12-hydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial (Secoferruginol) are diterpenes isolated from the heartwood of Cryptomeria japonica and are pharmacologically active substances. Dendritic cells (DC) have a key influence on the differentiation of naive T cells into Th1 or Th2 effector cells. We demonstrate that Sugiol and Secoferruginol activate human DC as documented by phenotypic and functional maturation and altered cytokine production. Human monocytes were exposed to Sugiol or Secoferruginol alone, or in combination with LPS and thereafter co-cultured with naive T cells. The expression levels of CD83 on Sugiol-primed DC were enhanced. Sugiol dose-dependently inhibited IL-12p70 production by LPS-primed DC and to a lesser extent, the production of the proinflammatory cytokines. Naive T cells co-cultured with Sugiol-primed DC, turned into typical Th2 which produced large quantities of IL-4 and released small amounts of IFN-γ and reduced Th1 cell polarizing capacity. Sugiol-primed DC induced the development of Th2 cells via the enhanced expression of OX40L and augmented the Th2 cell polarizing capacity of DC via the inhibition of IL-12p70. Similar results were obtained with Secoferruginol. These results suggest that some diterpenes modulate human DC function in a fashion that favors Th2 cell polarization and might have implication in autoimmune diseases. |
| Databáze: | OpenAIRE |
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