Myristic Acid, A Side Chain of Phorbol Myristate Acetate (PMA), Can Activate Human Polymorphonuclear Leukocytes to Produce Oxygen Radicals More Potently than PMA
| Autor: | Masahiro Kohno, Eiichiro Ichiishi, Mika Tada, Yoshimi Niwano, Natsumi Emoto, Rumiko Saito |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species Nutrition and Dietetics Superoxide Radical Clinical Biochemistry polymorphonuclear leukocyte Medicine (miscellaneous) Myristic acid chemistry.chemical_element food and beverages Oxygen law.invention chemistry.chemical_compound EPR spin-trapping Biochemistry chemistry law Saturated fatty acid Phorbol Original Article superoxide Electron paramagnetic resonance myristic acid phorbol 12-myristate 13-acetate |
| Zdroj: | Journal of Clinical Biochemistry and Nutrition |
| ISSN: | 1880-5086 0912-0009 |
| Popis: | Myristic acid (MyA), which is a saturated fatty acid (C14:0) and a side chain of phorbol 12-myristate 13-acetate (PMA), was examined if MyA stimulates human polymorphonuclear leukocytes (PMNs) to release oxygen radicals comparable to PMA by applying electron paramagnetic resonance (EPR)-spin-trapping method. When MyA was added to isolated human PMNs, spin adducts of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO)-OH and DMPO-OOH were time-dependently observed. The amounts of these spin adducts were larger than those of PMNs stimulated by PMA. These results clearly show that MyA is more potent agent to prime human PMNs than PMA, in a point of view of not only O(2) (.-) but also .OH production. This fact calls attention that too much intake of MyA that is known to be contained vegetable oils can lead to crippling effect through uncontrolled production of reactive oxygen species. |
| Databáze: | OpenAIRE |
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