Identification of stage-specifically expressed genes of Trichinella spiralis by suppression subtractive hybridization
| Autor: | Xue-Lin Wang, Q. J. Chen, Mingyuan Liu, D. Le Rhun, C. Y. Li, Xiuping Wu, Pascal Boireau, Baoquan Fu |
|---|---|
| Přispěvatelé: | Jilin University (JLU), Unité mixte de recherche biologie moléculaire et immunologie parasitaires et fongiques, Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Chinese Academy of Agricultural Sciences (CAAS), Swedish Institute for Infectious Disease Control, Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire - Alfort (ENVA)-Agence Française de Sécurité Sanitaire des Aliments (AFSSA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Proteases
DNA Complementary SUPPRESSION SUBTRACTIVE HYBRIDIZATION [SDV]Life Sciences [q-bio] Molecular Sequence Data Trichinella spiralis Retinoid X receptor Homology (biology) 030308 mycology & parasitology Mice 03 medical and health sciences Complementary DNA Animals Gene family Gene 030304 developmental biology Genetics Life Cycle Stages 0303 health sciences TRICHINELLA SPIRALIS STAGE-SPECIFICALLY EXPRESSED GENES biology Gene Expression Profiling Gene Expression Regulation Developmental Nucleic Acid Hybridization ADULTS biology.organism_classification Molecular biology Rats Infectious Diseases NEWBORN LARVAE Suppression subtractive hybridization Larva Animal Science and Zoology Parasitology |
| Zdroj: | Parasitology Parasitology, Cambridge University Press (CUP), 2007, 134, pp.1443-1455. ⟨10.1017/S0031182007002855⟩ |
| ISSN: | 0031-1820 1469-8161 |
| DOI: | 10.1017/S0031182007002855⟩ |
| Popis: | SUMMARYNewborn larvae (NBL) and adult (Ad) stage-specifically expressed genes or members of gene families ofTrichinella spiraliswere identified by suppression subtractive hybridization (SSH)†. Six cDNA clones were identified as NBL stage-specific, including 1 member of theT. spiralisgene family encoding glutamic acid-rich proteins, 2 clones encoding novel serine proteases, 2 closely related clones encoding proteins that are members of a deoxyribonuclease II (DNase II)-like family and 1 clone with no similarity to known genes. Four stage-specific clones encoding homologues of retinoid X receptor, caveolin, C2H2 type zinc finger protein and a putative protein with no homology to known sequences were obtained from 3-day-old adult worms. One gene specifically up-regulated in the 5-day-old adult worms encoding a putative cuticle collagen was also identified. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|