Noxious stimulation excites serotonergic neurons: A comparison between the lateral paragigantocellular reticular and the raphe magnus nuclei
| Autor: | Jean-François Bernard, Michel Hamon, Remi Gau, Caroline Sévoz-Couche |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Hot Temperature Biotin Fluorescent Antibody Technique Baroreflex Serotonergic Rats Sprague-Dawley Physical Stimulation Noxious stimulus Animals Nucleus raphe magnus Medulla Oblongata Behavior Animal Chemistry Iontophoresis Electrophysiological Phenomena Rats Electrophysiology Anesthesiology and Pain Medicine Nociception nervous system Neurology Receptive field Raphe Nuclei Neurology (clinical) Serotonin Neuroscience Serotonergic Neurons |
| Zdroj: | Pain. 154:647-659 |
| ISSN: | 0304-3959 |
| Popis: | The present study was designed to record electrophysiological responses to graded noxious thermal stimuli of serotonergic and nonserotonergic neurons in the lateral paragigantocellular reticular (LPGi) and the raphe magnus (RMg) nuclei in rats. All of the neurons recorded were juxtacellularly filled with neurobiotin and identified with double immunofluorescent labeling for both neurobiotin and serotonin. Under halothane anesthesia (0.75%), noxious thermal stimuli ⩾48°C activated almost all (88%) of the serotonergic neurons located within the LPGi (n=16). The increase in firing was clear (3.4±0.3spike/s: mean of responses above the population median) and sustained during the whole application of strong thermal noxious stimuli, with a high mean threshold (48.3±0.3°C) and large receptive fields. Recording of serotonergic neurons in the RMg (n=21) demonstrated that the proportion of strongly activated (>2spike/s) neurons (19% vs 59% for the LPGi) as well as the magnitude of the activation (2.1±0.4spike/s: mean of responses above the population median) to thermal noxious stimuli were significantly lower than in the LPGi (P |
| Databáze: | OpenAIRE |
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