FEATURES OF NITRIC OXIDE METABOLISM AND RISK OF DEVELOPING ENDOTHELIAL DYSFUNCTION IN CHILDREN WITH e-NOS GENE 4a/4b POLYMORPHISM UNDER LONG-TERM ENTERING 137Cs TO BODY
Autor: | I Ye Kolpakov, V V Vasylenko, V Yu Vdovenko, O M Leonovych, Ye I Stepanova, V M Zyhalo |
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Rok vydání: | 2018 |
Předmět: |
Male
medicine.medical_specialty Genotype Nitric Oxide Synthase Type III Gene Expression Radiation Dosage Nitric oxide FEV1/FVC ratio chemistry.chemical_compound Blood serum Gene Frequency Enos Risk Factors Internal medicine Radiation Ionizing medicine Humans Radiology Nuclear Medicine and imaging Vascular Diseases Endothelial dysfunction Allele Child Lung Alleles Polymorphism Genetic biology Bronchial Spasm business.industry Environmental Exposure Radiation Exposure biology.organism_classification medicine.disease Introns Endocrinology chemistry Chernobyl Nuclear Accident Cesium Radioisotopes Case-Control Studies Blood Vessels Female Gene polymorphism business Ukraine |
Zdroj: | Problemy radiatsiinoi medytsyny ta radiobiolohii. 23 |
ISSN: | 2304-8336 |
Popis: | To determine the features of the nitrogen oxide metabolism and risk of developing endothelial dysfunc-tion in children with e-NOS 4a/4b gene polymorphism, who live under prolonged enter 137Cs to the body.There were examined 117 children-residents of radioactively contaminated territories and50 children of control group. The level of stable metabolites was defined in blood serum (NO2- and NO3-). The ther-mographic method was used to register the endothelium dependent reaction of the vascular bed to changes in theblood supply. The ventilation capacity of the lungs was evaluated using this method of pneumotachography.Polymorphism in intron 4 of the gene e-NOS was studied by the method of polymerase chain reaction. The contentof 137Cs in the body of children was determined using a human radiation counter Skrynner M-3.In children-residents of radioactively contaminated territories with genotype 4a/4b com-paring to children who had genotype 4b/4b, the decrease in the nitric content of in the blood serum, the increase inthe thermographic index of the recovery period of blood circulation to the baseline level after occlusion test werenoted, that is indicative of the decreased NO-synthase active of vascular endothelium in the carriers of the minorallele a in the 4th intron of gene eNOS (genotype 4a/4b), and is a risk factor for development of endothelial dysfunc-tion. It was proved a decrease in the index of lung tissue elasticity and stretchability - FVC / NFVC of the lungs com-paring to children with genotype 4b/4b, there was a reduction of integral index of respiratory tract permeability -FEV1/NFEV1. The inverse correlation dependence between the presence of allele a in the genotype and the values ofFVC/NFVC of the lungs (r = -0.259; p0.05) and FEV1/NFEV1 (r = -0.2267; p0.05) was found. Signs of bron-chospasm were found in the carriers of the allele a in 1.5 times more often than in children-carriers of homozy-gotes from allele b.Meta. Vyznachyty osoblyvosti metabolizmu oksydu azotu ta ryzyk rozvytku endotelial'noï dysfunktsiï u diteĭ z4a/4b polimorfizmom gena e-NOS, iaki meshkaiut' za umov tryvalogo nadkhodzhennia 137Cs do organizmu.Materialy ta metody. Provedeno obstezhennia 117 diteĭ – meshkantsiv radioaktyvno zabrudnenykh terytoriĭ ta 50diteĭ kontrol'noï grupy. Vyznachaly riven' stabil'nykh metabolitiv u syrovattsi krovi (NO2- ta NO3-). Dliareiestratsiï endoteliĭzalezhnoï reaktsiï sudynnogo rusla na zminy umov krovopostachannia vykorystovuvaly ter-mografichnyĭ sposib. Ventyliatsiĭnu spromozhnist' legeniv otsiniuvaly za dopomogoiu metodu pnevmotakhografiï.Doslidzhennia polimorfizmu v 4-mu introni gena eNOS zdiĭsniuvaly metodom polimeraznoï lantsiugovoï reaktsiï.Vmist 137Cs v tili diteĭ vyznachaly za dopomogoiu lichyl'nyka vyprominiuvannia liudyny Skrynner-3M.Rezul'taty ta vysnovky. U diteĭ – meshkantsiv radioaktyvno zabrudnenykh terytoriĭ z genotypom 4a/4b u poriv-nianni z dit'my, iaki maly genotyp 4b/4b, vidmichalosia znyzhennia vmistu nitrytu v syrovattsi krovi, zbil'shennia ter-mografichnogo pokaznyka tryvalosti periodu vidnovlennia krovoobigu do vykhidnogo rivnia pislia okliuziĭnoï pro-by, shcho vkazuie na znyzhennia NO-syntaznoï aktyvnosti sudynnogo endoteliiu v osib-nosiïv minornogo alelia a v 4-muintroni gena eNOS (genotyp 4a/4b) ta ie faktorom ryzyku rozvytku endotelial'noï dysfunktsiï. Dovedeno zmen-shennia pokaznyka elastychnosti i roztiazhnosti legenevoï tkanyny – FZhIeL/NFZhIeL u diteĭ osnovnoï grupy z geno-typom 4a/4b v porivnianni z dit'my z genotypom 4b/4b, malo mistse takozh znyzhennia integral'nogo pokaznykaprokhidnosti dykhal'nykh shliakhiv – OFV1/NOFV1. Vyiavleno zvorotniu koreliatsiĭnu zalezhnist' mizh naiavnistiu aleliaa v genotypi ta velychynoiu pokaznykiv FZhIeL/NFZhIeL (r = -0,259; r -#060; 0,05) i OFV1/NOFV1 (r = -0,2627; r -#060; 0,05).Oznaky bronkhospazmu vyiavleni u nosiïv alelia a v 1,5 raza chastishe, nizh u diteĭ gomozygot z alelia b. |
Databáze: | OpenAIRE |
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