Removal of Urea, β2-Microglobulin, and Indoxyl Sulfate Assessed by Absorbance and Fluorescence in the Spent Dialysate During Hemodialysis
| Autor: | Ivo Fridolin, Merike Luman, Kai Lauri, Risto Tanner, Sigrid Kalle, Jana Holmar, Jürgen Arund |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_treatment Biomedical Engineering Biophysics Bioengineering 030204 cardiovascular system & hematology Proof of Concept Study Fluorescence Biomaterials Absorbance 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Renal Dialysis Dialysis Solutions medicine Humans Urea Dialysis Aged Chromatography Beta-2 microglobulin Chemistry Spectrum Analysis General Medicine Middle Aged Standard error 030228 respiratory system Indoxyl Sulfate Female Hemodialysis beta 2-Microglobulin Indican |
| Zdroj: | ASAIO journal (American Society for Artificial Internal Organs : 1992). 66(6) |
| ISSN: | 1538-943X |
| Popis: | In this study, simultaneous removal assessment of marker molecules from three uremic toxin groups was performed during different hemodialysis treatment modalities using optical characteristics of spent dialysate. Results from optical measurements were compared with the results from chemical laboratory. Ten chronic dialysis patients, mean age 59 ± 15 years, were included in the study during 40 hemodialysis sessions. Low-flux hemodialysis (HD), high-flux hemodialysis (HF), and postdilutional online hemodiafiltration (HDF) with different settings were used. The reduction ratio (RR) and total removed solute (TRS) of three uremic solutes were determined: small molecular weight urea, middle molecular β2-microglobulin (B2M), and protein-bound indoxyl sulfate (IS). Concentrations of these solutes in the spent dialysate were measured by laboratory (lab) and optical (opt) methods, in the serum by laboratory methods, and calculated RR values in percentage were compared accordingly. Total removed solute was obtained from the total dialysate collection (TDC) using lab and opt methods. The highest RR values were found for urea and B2M, and the lowest for IS. The difference between RR of lab and opt results estimated as mean accuracy (BIAS) was ≤8.1% for all three solutes. Good correspondence between TRS lab vs. opt was achieved, resulting in strong linear correlation values R from 0.727 for urea to 0.971 for IS. Accuracy for TRS values as BIAS ± standard error (SE), comparing lab vs. opt, showed no statistical difference for any of the observed uremic solutes (P > 0.05). The accuracy of the optical method was not influenced by the dialysis modality (HD, HF, and HDF). |
| Databáze: | OpenAIRE |
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