E-vapor aerosols do not compromise bone integrity relative to cigarette smoke after 6-month inhalation in an ApoE–/– mouse model
Autor: | Ee Tsin Wong, Victor Häussling, Patrice Leroy, Bjoern Titz, Manuel C. Peitsch, Romina H. Aspera-Werz, Wei Teck Tan, Justyna Szostak, Andreas K. Nussler, Christof Audretsch, Julia Hoeng, Peter Augat, Jenny Schaefer, Fabian Springer, K. Monica Lee, Arkadiusz K. Kuczaj, Marie K. Reumann, Sabrina Ehnert, Leticia Quentanilla-Fend, Manuela Martella, Andreas Badke |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Apolipoprotein E medicine.medical_specialty Time Factors Bone density Mice Knockout ApoE Health Toxicology and Mutagenesis Electronic Nicotine Delivery Systems Toxicology complex mixtures Bone and Bones Organ Toxicity and Mechanisms Cigarette Smoking Nicotine 03 medical and health sciences 0302 clinical medicine Internal medicine Smoke medicine Cigarette smoke Animals E-vapor aerosol Bone biomechanical properties Tobacco harm reduction Inhalation Exposure Inhalation Chemistry Vaping ApoE–/– mouse model General Medicine Bone structure X-Ray Microtomography Aerosol 030104 developmental biology medicine.anatomical_structure Endocrinology E-Cigarette Vapor 030220 oncology & carcinogenesis Cortical bone Female medicine.drug |
Zdroj: | Archives of Toxicology |
ISSN: | 1432-0738 0340-5761 |
Popis: | Cigarette smoke (CS) exposure is one of the leading risk factors for human health. Nicotine-containing inhalable products, such as e-cigarettes, can effectively support tobacco harm reduction approaches. However, there are limited comparative data on the effects of the aerosols generated from electronic vapor products (e-vapor) and CS on bone. Here, we report the effects of e-vapor aerosols and CS on bone morphology, structure, and strength in a 6-month inhalation study. Eight-week-old ApoE-/- mice were exposed to aerosols from three different e-vapor formulations-CARRIER (propylene glycol and vegetable glycerol), BASE (CARRIER and nicotine), TEST (BASE and flavor)-to CS from 3R4F reference cigarettes at matched nicotine concentrations (35 µg/L) or to fresh air (Sham) (N = 10 per group). Tibiae were analyzed for bone morphology by µCT imaging, biomechanics by three-point bending, and by histological analysis. CS inhalation caused a significant decrease in cortical and total bone volume fraction and bone density relative to e-vapor aerosols. Additionally, CS exposure caused a decrease in ultimate load and stiffness. In contrast, bone structural and biomechanical parameters were not significantly affected by e-vapor aerosol or Sham exposure. At the dissection time point, there was no significant difference in body weight or tibia bone weight or length among the groups. Histological findings revealed microcracks in cortical bone areas among all exposed groups compared to Sham control. In conclusion, because of the bone-preserving effect of e-vapor aerosols relative to CS exposure, e-vapor products could potentially constitute less harmful alternatives to cigarettes in situations in which bone health is of importance. |
Databáze: | OpenAIRE |
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