Characterization and antimicrobial susceptibility of one antibiotic-sensitive and one multidrug-resistant Corynebacterium kroppenstedtii strain isolated from patients with granulomatous mastitis
| Autor: | Fernández-Natal, I, Rodríguez-Lázaro, David, Marrodán-Ciordia, T, Saez-Nieto, Juan Antonio, Valdezate, Sylvia, Rodríguez-Pollán, H, Tauch, A, Soriano, F |
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| Přispěvatelé: | Junta de Castilla y León (España), Junta de Castilla y León |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Corynebacterium kroppenstedtii medicine.drug_class Tetracycline 030106 microbiology Antibiotics Biology Microbiología Antimicrobial susceptibility Microbiology lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine medicine MALDI-TOF MS lcsh:RC109-216 Etest Clindamycin biochemical phenomena metabolism and nutrition bacterial infections and mycoses Ciprofloxacin Multiple drug resistance Infectious Diseases 030220 oncology & carcinogenesis Vancomycin Gentamicin multidrug-resistant isolate medicine.drug granulomatous mastitis |
| Zdroj: | Repositorio Institucional de la Universidad de Burgos (RIUBU) instname Repisalud Instituto de Salud Carlos III (ISCIII) New Microbes and New Infections, Vol 14, Iss C, Pp 93-97 (2016) Europe PubMed Central |
| Popis: | Human infections associated with Corynebacterium kroppenstedtii are rarely reported, and this organism is usually described as antibiotic sensitive. Almost all published cases of C. kroppenstedtii infections have been associated with breast pathology in women and have been described in New Zealand, France, Canada, India and Japan. Here we describe the microbiologic characteristics of two strains isolated from two women diagnosed of granulomatous mastitis in Spain. One C. kroppenstedtii isolate was antibiotic sensitive while the other was multidrug resistant. Biochemical identification was possible using a wide battery of methods including API Coryne V2.0, API Strep, API NH, API NE, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene amplification and sequencing. Antimicrobial susceptibility to 28 antibiotics as determined by Etest showed one isolate being sensitive to benzylpenicillin, ciprofloxacin, moxifloxacin, gentamicin, vancomycin, clindamycin, tetracycline, linezolid and rifampin. The second isolate showed resistance to ciprofloxacin, moxifloxacin, clindamycin, tetracycline and rifampin. The multidrug-resistant isolate contained the erm(X), tet(W), cmx, aphA1-IAB, strAB and sul1 resistance genes known from the R plasmid pJA144188 of Corynebacterium resistens. These genes were absent in the genome of the antibiotic-sensitive isolate. This report confirms the tropism of this microorganism for women's breasts and presents the first description of a multidrug-resistant C. kroppenstedtii strain. Gerencia Regional de Salud, Junta de Castilla y León, Spain, research project GRS 698/A/2011 |
| Databáze: | OpenAIRE |
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