Quantitative Polymerase Chain Reaction Assay for Largemouth Bass Virus
| Autor: | S. G. Grimmett, Gregory A. Wooster, Geoffrey H. Groocock, Vanessa L. Schumacher, Paul R. Bowser, Rodman G. Getchell |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
food.ingredient
Ranavirus Micropterus Aquatic Science Biology Polymerase Chain Reaction Sensitivity and Specificity Virus Cell Line Fish Diseases chemistry.chemical_compound Bass (fish) food Plasmid Reference Values Animals Gene DNA Primers Reproducibility of Results biology.organism_classification Molecular biology DNA Virus Infections Real-time polymerase chain reaction chemistry Capsid Bass Capsid Proteins DNA |
| Zdroj: | Journal of Aquatic Animal Health. 19:226-233 |
| ISSN: | 1548-8667 0899-7659 |
| DOI: | 10.1577/h07-009.1 |
| Popis: | The use of quantitative polymerase chain reaction (QPCR) to test for largemouth bass virus (LMBV) was evaluated during a challenge experiment in which largemouth bass Micropterus salmoides were immersed in the type strain of LMBV. The real-time PCR and cell culture methods were both used to measure LMBV present in the inoculum. Additional samples tested by QPCR included gill, gonad, kidney, liver, mucus, spleen, and swim bladder. A plasmid clone containing a 248-base pair (bp) fragment of the major capsid protein gene (MCP*) was serially diluted and used as a standard to quantify the number of LMBV DNA copies present in the samples tested. A 62-bp fragment of DNA located in MCP* was amplified in the real-time PCR assay. This work has demonstrated the value of the QPCR assay in LMBV surveys. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text