Overexpression of p185 is not related to erbB2 amplification in ovarian cancer
| Autor: | Nicoletta Colombo, V. Lucchini, F. Morali, Sylvie Ménard, M. Cattabeni, Costantino Mangioni, L. Redaelli, M. Marzola, Manuela Campiglio, Maurizio D'Incalci, Elda Tagliabue |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Pathology
medicine.medical_specialty Receptor ErbB-2 Ovary Breast cancer Western blot Proto-Oncogene Proteins Proto-Oncogenes Gene duplication medicine Humans RNA Messenger Northern blot skin and connective tissue diseases Neoplasm Staging Ovarian Neoplasms Oncogene medicine.diagnostic_test business.industry Gene Amplification Hematology medicine.disease ErbB Receptors medicine.anatomical_structure Gene Expression Regulation Oncology Immunohistochemistry Female business Ovarian cancer |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0923-7534 |
| Popis: | Summary Background While in breast cancer the amplification and overexpression of the erbB2 gene has been reported in numerous studies and found to be correlated to poor prognosis, information about this oncogene with respect to ovarian cancer is still limited. A recent study reported that approximately 30% of tumor biopsies from ovarian cancer patients exhibited erbB2 amplification and overexpression and suggested that the overexpression of this oncogene is an indicator of bad prognosis in ovarian cancer. The purpose of our studies was to investigate amplification of the erbB2 gene, the levels of erbB2 m-RNA and the erbB2 product (pi85) in ovarian cancer, and the correlation between these findings and the pathological and clinical features. Results Amplification of the erbB2 gene was investigated by Southern blot analysis in 75 samples from 62 patients; mRNA levels were evaluated by Northern blot analysis in 58 samples from 48 patients; and pl85 was determined by immunohistochemistry in 65 samples from 65 patients. The erbB2 gene was amplified in only one case (1.6%), and a marked Increase in erbB2 mRNA was found only in the same case. Staining for pl85 was positive in 12 cases (18.5%). The staining was always confined to the cytoplasm except in the case that showed amplification of erbB2 in which pl85 was localized in the membrane. No correlation was found between pl85 positivity and pathological and clinical features or response to chemotherapy. Western blot analysis showed that the molecular weight of pl85 in positive ovarian cancer cells was approximately 10 KDa lower than in breast cancer. Conclusions In contrast to breast cancer, in ovarian cancer the amplification of erbB2 appears infrequent. The levels of pl85 detected by immunohistochemistry were not related to the amplification of the gene or to the increase in mRNA, suggesting the possibility of a longer half-life of the protein in the positive cases. The finding that erbB2 product in ovarian cancer is mostly localised in cytoplasm and not in the membrane as in breast cancer and that it has a lower molecular weight than the pl85 in breast cancer suggest that this oncogene plays a different biological role in these two neoplasms. |
| Databáze: | OpenAIRE |
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