Phenolic Assesment of Uncaria tomentosa L. (Cat’s Claw): Leaves, Stem, Bark and Wood Extracts
| Autor: | Renato Murillo Masis, Fernando Sánchez-Patán, María Monagas, Pedro J. Martín-Álvarez, Begoña Bartolomé, William Ramírez, Mirtha Navarro Hoyos |
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| Přispěvatelé: | Ministerio de Asuntos Exteriores y Cooperación (España), Fundación CRUSA, Consejo Superior de Investigaciones Científicas (España) |
| Rok vydání: | 2015 |
| Předmět: |
Hydroxybenzoic acid
Magnetic Resonance Spectroscopy Propelargonidins Flavonoid Pharmaceutical Science Article Analytical Chemistry lcsh:QD241-441 TQ-ESI/MS chemistry.chemical_compound Alkaloids Phenols lcsh:Organic chemistry Uncaria tomentosa L Drug Discovery Uncaria tomentosa Plant Bark Organic chemistry Proanthocyanidins Gallic acid Cat's Claw Physical and Theoretical Chemistry Flavonoids chemistry.chemical_classification 13C NMR UPLC Chromatography Plant Stems biology Plant Extracts Solid Phase Extraction Organic Chemistry biology.organism_classification 13C-NMR Plant Leaves alkaloids flavonoids proanthocyanidins propelargonidins chemistry Proanthocyanidin Chemistry (miscellaneous) visual_art visual_art.visual_art_medium Molecular Medicine Bark |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Molecules, Vol 20, Iss 12, Pp 22703-22717 (2015) Molecules; Volume 20; Issue 12; Pages: 22703-22717 Molecules Volume 20 Issue 12 Pages 22703-22717 |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules201219875 |
| Popis: | This article belongs to the Special Issue "Recent Advances in Plant Phenolics".-- This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license. The phenolic composition of extracts from Uncaria tomentosa L. from different regions of Costa Rica was studied using advanced analytical techniques such as UPLC/TQ-ESI-MS and 13C-NMR. Samples from leaves, stems, bark and wood (n = 22) were subjected to extraction to obtain phenolic and alkaloid extracts, separately. Comparatively, higher values of total phenolic content were observed for leaves, stems and bark (225–494 gallic acid equivalents/g) than for wood extracts (40–167 gallic acid equivalents/g). A total of 32 non-flavonoid and flavonoid compounds were identified in the phenolic extracts: hydroxybenzoic acids (benzoic, salicylic, 4-hydroxybenzoic, prochatechuic, gallic, syringic and vanillic acids), hydroxycinnamic acids (p-coumaric, caffeic, ferulic and isoferulic acids), flavan-3-ols monomers [(+)-catechin and (−)-epicatechin)], procyanidin dimers (B1, B2, B3, B4, B5, B7 and two other of unknown structure) and trimers (C1, T2 and one of unknown structure), flavalignans (four unknown structures pertaining to the cinchonain family) and propelargonidin dimers (four unknown structures, reported for the first time in U. tomentosa). Additionally, alkaloid extracts obtained from the plant residue after phenolic extraction exhibited a content of tetracyclic and pentacyclic alkaloids ranging between 95 and 275 mg/100 g of dry material for bark extracts, and between 30 and 704 mg/100 g for leaves extracts. In addition, a minor alkaloid was isolated and characterized, namely 18,19-dehydrocorynoxinoic acid. Our results confirmed the feasibility of U. tomentosa as a suitable raw material for obtaining phenolic- and alkaloid-rich extracts of potential interest. This project was partially funded by a grant from the Spanish International Development Cooperation Agency (AECID) (Refs. A/023397/09 and A/030037/10) and a joint grant from the Costa Rica-USA Foundation (CRUSA) and the Spanish Scientific Research Council (CSIC) (Ref. CR0024). We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI). |
| Databáze: | OpenAIRE |
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