Human papillomavirus status of head and neck cancer as determined in cytologic specimens using the hybrid-capture 2 assay
| Autor: | Kevin D. Frick, Jeremy D. Richmon, Belinda Akpeng, Justin A. Bishop, William H. Westra, Zhen Gooi, Takenori Ogawa, Patrick K. Ha, Diarmuid Coughlan, Sara I. Pai, David F. Smith, Christine G. Gourin, Zahra Maleki, Wayne M. Koch, Nishant Agrawal |
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| Rok vydání: | 2014 |
| Předmět: |
squamous cell carcinoma
Oncology Cancer Research medicine.medical_specialty Pathology oropharyngeal cancer oral-cancer Biopsy Fine-Needle Alphapapillomavirus survival Article Internal medicine Cytology medicine Humans Lymph node In Situ Hybridization risk hpv medicine.diagnostic_test business.industry tumor-origin fine needle aspiration Head and neck cancer association squamous-cell carcinoma medicine.disease Head and neck squamous-cell carcinoma Clinical trial medicine.anatomical_structure Fine-needle aspiration Head and Neck Neoplasms hybrid capture 2 Cervical lymph nodes DNA Viral cytology Immunohistochemistry head and neck cancer Lymph Nodes Oral Surgery business |
| Zdroj: | Oral Oncology. 50:600-604 |
| ISSN: | 1368-8375 |
| Popis: | Summary Objective A standardized assay to determine the HPV status of head and neck squamous cell carcinoma (HNSCC) specimens has not yet been established, particularly for cytologic samples. The goal of this study was to determine whether the hybrid capture-2 (HC-2) assay, already widely used for the detection of high risk HPV in cervical brushings, is applicable to cytologic specimens obtained from patients with suspected HNSCCs. Materials and methods Fine needle aspirates (FNA) of cervical lymph nodes were pre-operatively obtained from patients with suspected HNSCCs and evaluated for the presence of HPV using the HC-2 assay. HPV analysis was performed on the corresponding resected tissue specimens using p16 immunohistochemistry (IHC) and HR-HPV in situ hybridization (ISH). A cost analysis was performed using the Center for Medicare & Medicaid Services. Results HPV status of the cervical lymph node metastases was correctly classified using the HC-2 assay in 84% (21/25) of cases. Accuracy was improved to 100% when cytologic evaluation confirmed the presence of cancer cells in the test samples. The estimated cost savings to CMS using the HC-2 assay ranged from $113.74 to $364.63 per patient. Conclusions HC-2 is a reliable method for determining the HPV status of HNSCCs. Its application to HNSCCs may reduce costs by helping to localize the primary site during the diagnostic work-up as well as decrease the interval time of determining the HPV status which would be relevant for providing prognostic information to the patient as well as determining eligibility for clinical trials targeting this unique patient population. |
| Databáze: | OpenAIRE |
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