Diagnosis of herpes simplex virus-1 keratitis using Giemsa stain, immunofluorescence assay, and polymerase chain reaction assay on corneal scrapings
| Autor: | Prashant Garg, Savitri Sharma, S Athmanathan, Sesha B. Reddy, S Kaza, S Farhatullah |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent viruses Fluorescent Antibody Technique Herpesvirus 1 Human medicine.disease_cause Immunofluorescence Azure Stains Polymerase Chain Reaction Sensitivity and Specificity Giemsa stain Keratitis law.invention Cellular and Molecular Neuroscience Predictive Value of Tests law parasitic diseases Humans Medicine Child Antigens Viral Polymerase chain reaction Aged Aged 80 and over Laboratory Science - Scientific Reports medicine.diagnostic_test biology business.industry Infant Middle Aged biology.organism_classification medicine.disease corneal ulcer Virology Sensory Systems Acanthamoeba Ophthalmology Herpes simplex virus Giant cell Child Preschool DNA Viral Keratitis Herpetic Female business |
| Zdroj: | British Journal of Ophthalmology. 88:142-144 |
| ISSN: | 0007-1161 |
| DOI: | 10.1136/bjo.88.1.142 |
| Popis: | Aims: To evaluate three tests used routinely for the diagnosis of herpes simplex virus (HSV) keratitis. Methods: Corneal scrapings from 28 patients with clinically typical dendritic corneal ulcer suggestive of HSV keratitis, and 30 patients with clinically non-viral corneal ulcers, were tested by (i) Giemsa stain for multinucleated giant cells, (ii) immunofluorescence assay (IFA) for HSV-1 antigen, and (iii) polymerase chain reaction (PCR) for HSV-1 DNA, by investigators masked to clinical diagnosis. The control subjects were also investigated by smears and cultures for bacteria, fungus, and Acanthamoeba. Results: The specificity and positive predictive values of all three tests for the diagnosis of HSV keratitis were between 95–100%. The sensitivity of IFA and PCR was 78.6% and 81.2%, respectively, and the difference was not significant; however, their sensitivity and negative predictive value were significantly higher than Giemsa stain. Conclusions: While a combination of IFA and PCR constitute the choice of tests in clinically suspected cases of HSV keratitis, multinucleated giant cells in Giemsa stain can pre-empt testing by IFA and PCR in otherwise atypical cases of HSV keratitis. |
| Databáze: | OpenAIRE |
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