Analysis of glycosylinositol phosphorylceramides expressed by the opportunistic mycopathogen Aspergillus fumigatus
| ISSN: | 0022-2275 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b59548fcb6d916ca6d453fa2c2c9f41 https://pubmed.ncbi.nlm.nih.gov/17488996 |
| Rights: | OPEN |
| Přírůstkové číslo: | edsair.doi.dedup.....7b59548fcb6d916ca6d453fa2c2c9f41 |
| Autor: | Chaeho Park, Marcos S. Toledo, Beau Bennion, Sherry A. Castle, Steven B. Levery, Anita H. Straus, Luciana Lopes Guimarães, Helio K. Takahashi, Michelle Momany, Rebecca Lindsey |
| Rok vydání: | 2007 |
| Předmět: |
Stereochemistry
Glycosylphosphatidylinositol Molecular Sequence Data electrospray ionization QD415-436 Biochemistry Glycosphingolipids Aspergillus fumigatus Microbiology chemistry.chemical_compound Endocrinology Glycolipid galactofuranose Murine monoclonal antibody chemistry.chemical_classification Paracoccidioides brasiliensis biology fungus Monosaccharides Antibodies Monoclonal Cell Biology Acidic Glycosphingolipids biology.organism_classification Enzyme chemistry Carbohydrate Sequence monoclonal antibody Phosphodiester bond sphingolipid glycolipid |
| Zdroj: | Journal of Lipid Research, Vol 48, Iss 8, Pp 1801-1824 (2007) |
| ISSN: | 0022-2275 |
| Popis: | Acidic glycosphingolipid components were extracted from the opportunistic mycopathogen Aspergillus fumigatus and identified as inositol phosphorylceramide and glycosylinositol phosphorylceramides (GIPCs). Using nuclear magnetic resonance sppectroscopy, mass spectrometry, and other techniques, the structures of six major components were elucidated as Ins-P-Cer (Af-0), Manp(alpha1-->3)Manp(alpha1-->2)Ins-P-Cer (Af-2), Manp(alpha1-->2)Manp(alpha1-->3)Manp(alpha1-->2)Ins-P-Cer (Af-3a), Manp(alpha1-->3)[Galf(beta1-->6)]Manp(alpha1-->2)-Ins-P-Cer (Af-3b), Manp(alpha1-->2)-Manp(alpha1-->3)[Galf(beta1-->6)]Manp(alpha1-->2)Ins-P-Cer (Af-4), and Manp(alpha1-->3)Manp(alpha1-->6)GlcpN(alpha1-->2)Ins-P-Cer (Af-3c) (where Ins = myo-inositol and P = phosphodiester). A minor A. fumigatus GIPC was also identified as the N-acetylated version of Af-3c (Af-3c*), which suggests that formation of the GlcNalpha1-->2Ins linkage may proceed by a two-step process, similar to the GlcNalpha1-->6Ins linkage in glycosylphosphatidylinositol (GPI) anchors (transfer of GlcNAc, followed by enzymatic de-N-acetylation). The glycosylinositol of Af-3b, which bears a distinctive branching Galf(beta1-->6) residue, is identical to that of a GIPC isolated previously from the dimorphic mycopathogen Paracoccidioides brasiliensis (designated Pb-3), but components Af-3a and Af-4 have novel structures. Overlay immunostaining of A. fumigatus GIPCs separated on thin-layer chromatograms was used to assess their reactivity against sera from a patient with aspergillosis and against a murine monoclonal antibody (MEST-1) shown previously to react with the Galf(beta1-->6) residue in Pb-3. These results are discussed in relation to pathogenicity and potential approaches to the immunodiagnosis of A. fumigatus. |
| Databáze: | OpenAIRE |
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