Identification of a liver-specific cAMP response element in the human argininosuccinate synthetase gene
| Autor: | Chun-Ping Yang, Tai-Ru Guei, Tsung-Sheng Su, Mei-Chun Liu |
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| Rok vydání: | 2008 |
| Předmět: |
Response element
Argininosuccinate synthase Biophysics Argininosuccinate Synthase Response Elements CREB Biochemistry Gene Expression Regulation Enzymologic Transcription (biology) Gene expression Transcriptional regulation Deoxyribonuclease I Humans Cyclic AMP Response Element-Binding Protein Molecular Biology Gene Base Sequence biology DNA Sequence Analysis DNA Cell Biology Physical Chromosome Mapping Molecular biology Liver biology.protein CREB1 |
| Zdroj: | Biochemical and Biophysical Research Communications. 377:257-261 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2008.09.118 |
| Popis: | Argininosuccinate synthetase (ASS), a key enzyme in the urea cycle, participates in many metabolic processes including arginine biosynthesis and the citrulline-nitric oxide (NO) cycle. Factors like diets, hormones and pro-inflammatory stimuli are known to regulate ASS gene expression primarily at the transcription level. However, little is known about the cis-elements for transcriptional regulation of the ASS gene. In this study, we employed DNase I hypersensitive sites mapping to identify potential regulatory sites of the gene and revealed a site located at 10 kb upstream of the transcription start site which is responsible for liver-specific cAMP induction. Furthermore, a cAMP response element (CRE) highly conserved among mammals was identified and was experimentally verified. Our results show that liver-specific enhancement of ASS gene expression is mediated in part by the cAMP signaling pathway through a distal CRE site. |
| Databáze: | OpenAIRE |
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