Influence of serum percentage on the behavior of Wharton's jelly mesenchymal stem cells in culture
| Autor: | P Labrude, C Harmouch, R El-Omar, Véronique Decot, Halima Kerdjoudj, Patrick Menu |
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| Přispěvatelé: | Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Middle East Institute of Health, Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Biomatériaux et inflammation en site osseux - EA 4691 (BIOS), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Vascular Endothelial Growth Factor A
MESH: Epidermal Growth Factor Polymers Cell MESH: Insulin-Like Growth Factor I Cell Culture Techniques MESH: Spheroids Cellular Biocompatible Materials Cell Count MESH: Flow Cytometry MESH: Tissue Engineering 0302 clinical medicine MESH: Cell Aggregation Coated Materials Biocompatible MESH: Biocompatible Materials MESH: Coated Materials Biocompatible Wharton's jelly Polyamines Insulin-Like Growth Factor I Cell Aggregation 0303 health sciences medicine.diagnostic_test Chemistry MESH: Polystyrenes General Medicine Flow Cytometry Polyelectrolytes 3. Good health MESH: Polymers Blood Phenotype medicine.anatomical_structure Fibroblast Growth Factor 2 Biomedical Engineering MESH: Phenotype Flow cytometry Biomaterials 03 medical and health sciences Cations Spheroids Cellular medicine MESH: Blood Humans MESH: Cell Shape [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] MESH: Cations Cell Shape MESH: Polyamines 030304 developmental biology MESH: Cell Culture Techniques MESH: Humans Epidermal Growth Factor Tissue Engineering MESH: Fibroblast Growth Factor 2 MESH: Cell Count MESH: Vascular Endothelial Growth Factor A Mesenchymal stem cell Spheroid Mesenchymal Stem Cells [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Fibroblasts Chondrogenesis Molecular biology Culture Media MESH: Fibroblasts MESH: Culture Media MESH: Mesenchymal Stromal Cells Polystyrenes Fetal bovine serum [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology 030215 immunology Explant culture |
| Zdroj: | Bio-Medical Materials and Engineering Bio-Medical Materials and Engineering, IOS Press, 2013, 23 (4), pp.273-280. ⟨10.3233/BME-130751⟩ |
| ISSN: | 0959-2989 1878-3619 |
| DOI: | 10.3233/BME-130751⟩ |
| Popis: | International audience; Mesenchymal stem cells (MSCs) are multipotent cells able to differentiate into several lineages with valuable applications in regenerative medicine. MSCs differentiation is highly dependent on physicochemical properties of the culture substrate, cell density and on culture medium composition.In this study, we assessed the influence of fetal bovine serum (FBS) level on Wharton's jelly (WJ)-MSCs behavior seeded on polyelectrolyte multilayer films (PEMF) made of four bilayers of poly-allylamine hydrochloride (PAH) as polycation and poly-styrene sulfonate (PSS) as polyanion.MSCs isolated from WJ by explants method were amplified until the third passage. Their phenotypic characterization was performed by flow cytometry analyses. MSCs were seeded on PEMF, in Endothelial growth medium-2 (EGM-2) supplemented by either 5% or 2% FBS. Cell's behavior was monitored for 20 days by optical microscopy and immunofluorescence.Until 2 weeks on glass slides, no difference was observed whatever the FBS percentage. Then with 5% FBS, MSCs formed three-dimensional spheroids on PSS/PAH after 20 days of culture with a nuclear aggregate. Whereas, with 2% FBS, these spheroids did not appear and cells grown in 2D conserved the fibroblast-like morphology.The decrease of FBS percentage from 5% to 2% avoids 3D cell spheroids formation on PAH/PSS. Such results could guide bioengineering towards building 2D structures like cell layers or 3D structures by increasing the osteogenic or chondrogenic differentiation potential of MSCs. |
| Databáze: | OpenAIRE |
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