Evolution of l ‐ DOPA 4,5‐dioxygenase activity allows for recurrent specialisation to betalain pigmentation in Caryophyllales
| Autor: | Boas Pucker, Lijun Zhao, John C. Cushman, Helene Tiley, Alfonso Timoneda, Samuel Lopez-Nieves, Michael J. Moore, Won Cheol Yim, Michael J. Sanderson, Stephen A. Smith, Hester Sheehan, Samuel F. Brockington, Tao Feng, Nathanael Walker-Hale, Dario Copetti, Roshani Badgami, Rui Guo |
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| Přispěvatelé: | University of Zurich, Brockington, Samuel F, Sheehan, Hester [0000-0002-2169-5206], Feng, Tao [0000-0002-0489-2021], Walker-Hale, Nathanael [0000-0003-1105-5069], Lopez-Nieves, Samuel [0000-0002-3583-0392], Pucker, Boas [0000-0002-3321-7471], Guo, Rui [0000-0002-5165-7905], Yim, Won C [0000-0002-7489-0435], Badgami, Roshani [0000-0002-9290-3420], Timoneda, Alfonso [0000-0002-7024-8947], Zhao, Lijun [0000-0001-7317-830X], Tiley, Helene [0000-0002-4227-1824], Copetti, Dario [0000-0002-2680-2568], Sanderson, Michael J [0000-0002-0855-9648], Cushman, John C [0000-0002-5561-1752], Moore, Michael J [0000-0003-2222-8332], Smith, Stephen A [0000-0003-2035-9531], Brockington, Samuel F [0000-0003-1216-219X], Apollo - University of Cambridge Repository |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine 5-dioxygenase (DODA) Lineage (genetic) Physiology Context (language use) plant pigments Plant Science Biology 01 natural sciences Dioxygenases Levodopa 10127 Institute of Evolutionary Biology and Environmental Studies 03 medical and health sciences Phylogenetics Convergent evolution 1110 Plant Science Gene duplication 5‐dioxygenase (DODA) convergent evolution metabolic operon Gene l-DOPA 4 5-dioxygenase (DODA) Phylogeny Caryophyllales Pigmentation Dioxygenase activity gene duplication l-DOPA 4 1314 Physiology biology.organism_classification anthocyanins 030104 developmental biology Evolutionary biology betalains specialised metabolism metabolic� operon 570 Life sciences biology 590 Animals (Zoology) l‐DOPA 4 010606 plant biology & botany |
| Zdroj: | New Phytologist, 227 (3) |
| ISSN: | 1469-8137 0028-646X |
| Popis: | • The evolution of l ‐DOPA 4,5‐dioxygenase activity, encoded by the gene DODA , was a key step in the origin of betalain biosynthesis in Caryophyllales. We previously proposed that l ‐DOPA 4,5‐dioxygenase activity evolved via a single Caryophyllales‐specific neofunctionalisation event within the DODA gene lineage. However, this neofunctionalisation event has not been confirmed and the DODA gene lineage exhibits numerous gene duplication events, whose evolutionary significance is unclear. • To address this, we functionally characterised 23 distinct DODA proteins for l ‐DOPA 4,5‐dioxygenase activity, from four betalain‐pigmented and five anthocyanin‐pigmented species, representing key evolutionary transitions across Caryophyllales. By mapping these functional data to an updated DODA phylogeny, we then explored the evolution of l ‐DOPA 4,5‐dioxygenase activity. • We find that low l ‐DOPA 4,5‐dioxygenase activity is distributed across the DODA gene lineage. In this context, repeated gene duplication events within the DODA gene lineage give rise to polyphyletic occurrences of elevated l ‐DOPA 4,5‐dioxygenase activity, accompanied by convergent shifts in key functional residues and distinct genomic patterns of micro‐synteny. • In the context of an updated organismal phylogeny and newly inferred pigment reconstructions, we argue that repeated convergent acquisition of elevated l ‐DOPA 4,5‐dioxygenase activity is consistent with recurrent specialisation to betalain synthesis in Caryophyllales. New Phytologist, 227 (3) ISSN:0028-646X ISSN:1469-8137 |
| Databáze: | OpenAIRE |
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