Plasma polymerization-modified bacterial polyhydroxybutyrate nanofibrillar scaffolds
| Autor: | Melike Erol-Demirbilek, Emir Baki Denkbaş, Zeynep Karahaliloğlu, Mesut Şam, Murat Demirbilek, Necdet Sağlam |
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| Přispěvatelé: | Sabire Yazıcı Fen Edebiyat Fakültesi, SAM, Mesut -- 0000-0002-6429-7144, SAGLAM, NECDET -- 0000-0002-5463-8355 |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Materials science
Polymers and Plastics Polyesters technology industry and agriculture macromolecular substances General Chemistry Polyethylene glycol Nanofiber Biodegradable polymer Electrospinning Plasma polymerization Surfaces Coatings and Films Polyhydroxybutyrate Polyester chemistry.chemical_compound Tissue engineering Chemical engineering chemistry Biopolymers and Renewable Polymers Polymer chemistry Materials Chemistry Biocompatibility |
| Popis: | WOS: 000314927200069 The design and the development of novel scaffold materials for tissue engineering have attracted much interest in recent years. Especially, the prepared nanofibrillar scaffold materials from biocompatible and biodegradable polymers by electrospinning are promising materials to be used in biomedical applications. In this study, we propose to produce low-cost and cell-friendly bacterial electrospun PHB polymeric scaffolds by using Alcaligenes eutrophus DSM 545 strain to PHB production. The produced PHB was characterized by Nuclear Magnetic Resonance (NMR) and Fourier Transform Infrared Spectroscopy (FTIR). Nanofibrous scaffolds were fabricated via electrospinning method that has a fiber diameter approximately 700800 nm. To investigate cell attachment, cell growth, and antioxidant enzyme activity on positively and negatively charged PHB scaffold, PHB surface was modified by plasma polymerization technique using polyethylene glycol (PEG) and ethylenediamine (EDA). According to the results of superoxide dismutase (SOD) activity study, PEG-modified nanofibrillar scaffolds indicated more cellular resistance against oxidative stress compared to the EDA modification. As can be seen in cell proliferation results, EDA modification enhanced the cell proliferation more than PEG modification, while PEG modification is better as compared with nonmodified scaffolds. In general, through plasma polymerization technique, surface modified nanofibrillar structures are effective substrates for cell attachment and outgrowth. (c) 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013 Hacettepe University, Scientific Researches Unit This study was carried out under the auspices of the Hacettepe University, Scientific Researches Unit. The authors would like to thank Koray Mizrak and Cem Bayram for their help in SEM and XPS characterizations. |
| Databáze: | OpenAIRE |
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