Endobronchial Ultrasound-Guided Radiofrequency Ablation of Lung Tumors and Mediastinal Lymph Nodes: A Preclinical Study in Animal Lung Tumor and Mediastinal Adenopathy Models
| Autor: | Hideki Ujiie, Yamato Motooka, Tsukasa Ishiwata, Tomonari Kinoshita, Nicholas Bernards, Kosuke Fujino, Harley Chan, Terunaga Inage, Tatsuya Kato, Alexander Gregor, Makoto Suzuki, Kazuhiro Yasufuku |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Lung Neoplasms Radiofrequency ablation medicine.medical_treatment Sus scrofa Plasma Cell Granuloma Pulmonary 030204 cardiovascular system & hematology Endosonography law.invention 03 medical and health sciences 0302 clinical medicine Bronchoscopy law Cell Line Tumor medicine Animals Lung cancer Electrodes Lymph node Ultrasonography Interventional Radiofrequency Ablation Lung medicine.diagnostic_test business.industry Mediastinum Neoplasms Experimental General Medicine Ablation medicine.disease Bronchoscopes medicine.anatomical_structure 030228 respiratory system Lymphatic Metastasis Feasibility Studies Lymph Node Excision Surgery Histopathology Lymph Nodes Rabbits Lymph Radiology Cardiology and Cardiovascular Medicine business |
| Zdroj: | Seminars in Thoracic and Cardiovascular Surgery. 32:570-578 |
| ISSN: | 1043-0679 |
| DOI: | 10.1053/j.semtcvs.2020.02.003 |
| Popis: | Radiofrequency ablation (RFA) can be a therapeutic option in medically inoperable lung cancer patients. In this study, we evaluated a prototype bipolar RFA device applicator that can be deployed from a standard endobronchial ultrasound (EBUS) bronchoscope to determine feasibility and histopathological analysis in animal models. Rabbit lung cancers were created by transbronchial injection of VX2 rabbit cancer cells. Once the tumors were developed, they were ablated transpleurally, under EBUS guidance using the prototype RFA device. The animals were then sacrificed for specimen resection. Pig inflammatory lung pseudo-tumors and lymphadenopathy were created by transbronchial injection of a talc paste and ablated transbronchially under EBUS guidance. Pigs were evaluated at 5 days, 2 weeks, and 4 weeks following ablation by bronchoscopy and cone beam computed tomography before necropsy. Nicotinamide adenine dinucleotide hydrogen diaphorase staining was employed to measure the ablation area. Twenty-four VX2 rabbit tumors were ablated. The total ablated area ranged from 0.6 to 3.0 cm2 (mean: 1.8 cm2), corresponding to a total energy range of 1 to 6 kJ. Six pig lung pseudo-tumors and 5 mediastinal lymph nodes were ablated. Adjacent airway ulceration was observed in 3 ablations of lymph nodes. These airway complications resolved within 4 weeks of RFA without any treatment. There was no hemoptysis, air embolism, respiratory distress, or other serious complication noted. In these 2 animal models, we provide evidence that EBUS-guided bipolar RFA is feasible and histopathology shows that can ablate lung tumors and mediastinal lymph nodes under real-time ultrasound guidance. |
| Databáze: | OpenAIRE |
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