DNA Polymerases of Myeloma: Template and Primer Specificities of Two Enzymes
| Autor: | Francis J. Persico, A. Arthur Gottlieb |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
DNA polymerase
DNA polymerase II Polynucleotides Oligonucleotides Tritium General Biochemistry Genetics and Molecular Biology Mice Adenosine Triphosphate Animals Thymine Nucleotides Chromatography DNA clamp biology Adenine Nucleotides Chemistry DNA replication DNA DNA Neoplasm Templates Genetic General Medicine Molecular biology DNA Nucleotidyltransferases biology.protein Primase Primer (molecular biology) DNA polymerase I Multiple Myeloma DNA polymerase mu |
| Zdroj: | Nature New Biology. 239:173-176 |
| ISSN: | 2058-1092 0090-0028 |
| DOI: | 10.1038/newbio239173a0 |
| Popis: | INTEREST in the viral RNA-dependent DNA polymerases1,2 has led to an examination of mammalian DNA polymerases and to the characterization in certain systems of DNA polymerases which can transcribe ribopolymer chains of appropriate ribopolymer-oligodeoxyribopolymer hybrids3,4. The nature of these enzymes and their function in cells are unclear. We have reported the separation, by chromatography on DEAE-cellulose, of the DNA polymerases of the murine myeloma tumour into two separate fractions which we have designated as fractions D-I and D-II (ref. 5). Each of these fractions contains an activity capable of utilizing activated DNA and of transcribing the ribopolymer strand of poly rA oligo dT. We describe here the separation of two DNA polymerase enzymes in fraction D-I. |
| Databáze: | OpenAIRE |
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