Stimulation of in-vitro angiogenesis by low concentrations of risedronate is mitigated by 1,25-dihydroxyvitamin D3 or 24,25-dihydroxyvitamin D3
| Autor: | Cristina Navarro-Valverde, Raquel Santiago-Mora, Antonio Casado-Díaz, Gabriel Dorado, José Manuel Quesada-Gómez |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
24 25-Dihydroxyvitamin D 3 Angiogenesis Endocrinology Diabetes and Metabolism Clinical Biochemistry Neovascularization Physiologic Stimulation In Vitro Techniques Biochemistry Endocrinology Calcitriol Internal medicine medicine Vitamin D and neurology Animals Humans Molecular Biology Tube formation Matrigel Bone Density Conservation Agents Chemistry Etidronic Acid Cell migration Chemotaxis Cell Biology In vitro Molecular Medicine Risedronic Acid |
| Zdroj: | The Journal of Steroid Biochemistry and Molecular Biology. 148:214-218 |
| ISSN: | 0960-0760 |
| DOI: | 10.1016/j.jsbmb.2014.10.018 |
| Popis: | The nitrogen-containing or nitrogenous bisphosphonates (N-BF) are currently the main class of drugs used for the treatment of diseases characterized by an increased bone resorption. Preliminary data suggest that N-BF have also direct or indirect anti-tumoral effects, and recent evidence suggests that part of the anti-tumoral activity of N-BF may be attributed to their anti-angiogenic capacity when they are used at high concentrations. On the other hand, an optimal vitamin-D status seems to be necessary to maximize the bone response to N-BF. Our aim has been to evaluate the effect of risedronate, alone or in combination with either 1,25(OH)2D3 or 24,25(OH)2D3 (two main vitamin-D metabolites) on parameters related to the angiogenic capacity of human umbilical-vein endothelial cells (HUVEC). The studies of tube formation through in-vitro angiogenesis assays with Matrigel, chemotaxis and migration in a scratch assay showed that low concentrations of risedronate (0.01 to 1 μM) stimulated angiogenesis and cellular migration in vitro. The presence of 1,25(OH)2D3 in the medium inhibited tubular-structure formation and cellular migration. In addition, the presence of 1,25 or 24,25(OH)2D3 in the culture medium also decreased the pro-angiogenic effects of low-concentrations of risedronate. These data show the differential effects of different concentrations of vitamin-D metabolites and risedronate on angiogenesis, thus stressing the importance of an adequate vitamin D status during medical treatment with risedronate. This article is part of a Special Issue entitled ‘17th Vitamin D Workshop’. |
| Databáze: | OpenAIRE |
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