Possible link between glycolysis and apoptosis induced by sodium fluoride
| Autor: | Hiroshi Sakagami, Kanji Sugiyama, Shahead Ali Chowdhury, Osamu Amano, Fumitoshi Takayama, Kiso Akahane, Sufi Reza M D Morshed, Sumiko Otsuki, Yoshiko Yokote, K. Hashimoto, Takao Satoh, Toshikazu Yasui |
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| Rok vydání: | 2005 |
| Předmět: |
Glutamine
Apoptosis HL-60 Cells Biology Statistics Nonparametric 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Sodium fluoride Humans Glycolysis Cytotoxicity General Dentistry Membrane potential 030206 dentistry Hypoxia-Inducible Factor 1 alpha Subunit Cariostatic Agents Cell biology Glucose chemistry Biochemistry Gene Expression Regulation 030220 oncology & carcinogenesis Caspases DNA fragmentation Sodium Fluoride bcl-Associated Death Protein Fluoride |
| Zdroj: | Journal of dental research. 84(10) |
| ISSN: | 0022-0345 |
| Popis: | Fluoride has been used to prevent caries in the dentition, but the possible underlying mechanisms of cytotoxicity induction by this compound are still unclear. Since fluoride is known as an inhibitor of glycolytic enzymes, we investigated the possible connection between NaF-induced apoptosis and glycolysis in human promyelocytic leukemia HL-60 cells. NaF-induced apoptotic cell death is characterized by caspase activation, internucleosomal DNA fragmentation, loss of mitochondrial membrane potential, and production of apoptotic bodies. Higher activation of caspases-3 and -9, as compared with that of caspase-8, suggested the involvement of an extrinsic pathway. Utilization of glucose was nearly halted by NaF, whereas that of glutamine was rather enhanced. NaF enhanced the expression of Bad protein, but not that of Bcl-2 and Bax proteins, and reduced HIF-1α mRNA expression. Analysis of these data suggests a possible link between glycolysis and apoptosis. |
| Databáze: | OpenAIRE |
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