A Novel Human Acute Encephalitis Caused by Pseudorabies Virus Variant Strain
| Autor: | Xiangru Wang, Liping Zhang, Qingyun Liu, Li Xing, Ban Fuguo, Wei Li, Lingzhi Qin, Bin Wu, Shibang Guo, Shifang Ding, Wang Dongfang, Caihua Xie, Huanchun Chen, Ruoqian Yan, Xiaojuan Wang, Jiewen Zhang, Haichang Ma, Changxian Dong, Hongna Yang, Lingxiao Feng, Wang Cui, Jixuan Li |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Swine animal diseases viruses 030106 microbiology Pseudorabies Virus 03 medical and health sciences medicine Animals Humans Neutralizing antibody Pathogen Phylogeny chemistry.chemical_classification Swine Diseases biology business.industry Strain (biology) medicine.disease biology.organism_classification Virology Herpesvirus 1 Suid 030104 developmental biology Infectious Diseases chemistry biology.protein Encephalitis Antibody business Glycoprotein |
| Zdroj: | Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 73(11) |
| ISSN: | 1537-6591 |
| Popis: | Background Pseudorabies virus (PRV) is a common pathogen in multiple animal species, particularly in pigs. However, PRV infection in humans is rare and, to the best of our knowledge, PRV has never been isolated from human cases before. Methods Four acute encephalitis cases in humans were confirmed as PRV infection based on clinical symptoms, laboratory diagnosis, and metagenomic next-generation sequencing (mNGS). Cerebrospinal fluid (CSF) samples were collected and applied for virus isolation. Etiological and genetic characteristics of this PRV human isolate were further determined. Results The patients manifested respiratory dysfunction and acute neurological symptoms. The mNGS revealed PRV-specific nucleotide sequences in patients’ CSF samples (7–6198 reads and 0.2446%–80.58% coverage). The PRV envelope glycoprotein B antibody, glycoprotein E antibody, and neutralizing antibody were positively detected. For the first time, a PRV strain, designated hSD-1/2019, was isolated and identified from a CSF sample, and transmission electron microscopy revealed that hSD-1/2019 had typical morphology similar to that of swine PRV. Phylogenetic analysis illustrated that hSD-1/2019 was genetically closest to those PRV variant strains currently circulating in pigs in China, and this strain showed similar etiological characteristics to Chinese PRV variant strains, while different from Chinese classical strain. Moreover, hSD-1/2019 showed high pathogenicity and induced acute neurological symptoms in pigs. Conclusions A PRV strain was isolated from an acute human encephalitis case. This isolate showed close phylogenetic relationships and similar etiological characteristics to Chinese PRV variant strains, implying the great risk of PRV transmission from pigs to humans. |
| Databáze: | OpenAIRE |
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