Alpha-1 Acid Glycoprotein and Podocin mRNA as Novel Biomarkers for Early Glomerular Injury in Obese Children
| Autor: | Marcelina Żabińska, Joanna Chrzanowska, Anna Medyńska, Danuta Zwolińska, Dorota Bartoszek, Katarzyna Kościelska-Kasprzak |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
obesity urinary mRNA expression of podocyte-associated proteins Urinary system Article Podocyte Nephrin Glomerulopathy Internal medicine medicine biology business.industry General Medicine medicine.disease glomerular injury medicine.anatomical_structure Endocrinology Albuminuria Podocin biology.protein Biomarker (medicine) Medicine Synaptopodin medicine.symptom business alpha-1 acid glycoprotein |
| Zdroj: | Journal of Clinical Medicine, Vol 10, Iss 4129, p 4129 (2021) Journal of Clinical Medicine Volume 10 Issue 18 |
| ISSN: | 2077-0383 |
| Popis: | Introduction: Obesity, which is a serious problem in children, has a negative impact on many organs, including kidneys, and obesity-related glomerulopathy (ORG) is an increasingly common cause of ESKD (end-stage kidney disease) in adults. Early-detected and -treated glomerular lesions are reversible, so it is important to find a useful marker of early damage. The study aimed to evaluate the albumin-to-creatinine ratio (ACR), urinary alpha-1-acid glycoprotein (α1-AGP), and mRNA of podocyte-specific proteins as indicators of glomerular injury and their relationship with the degree of obesity and metabolic disorders. Materials and Methods: A total of 125 obese children and 33 healthy peers were enrolled. Patients were divided into two groups, depending on SDS BMI values. ACR, α1-AGP, mRNA expression of nephrin, synaptopodin, podocin, and C2AP protein in urine sediment were measured. Results: ACR values did not differ between groups and were within the normal range. α1-AGP and mRNA expression were significantly higher in obese children compared with controls. mRNA expression of the remaining podocyte proteins was similar in both groups. No significant differences concerning all examined parameters were found depending on the degree of obesity. There was a positive significant correlation between α1-AGP and ACR. Conclusions: Increased α1-AGP before the onset of albuminuria suggests its usefulness as a biomarker of early glomerular damage in obese children. An increased podocin mRNA expression also indicates podocyte damage and may be linked to ORG development. The lack of increase in expression of other podocyte proteins suggests that podocin mRNA may be a more specific and sensitive biomarker. The degree of obesity has no impact on the tested parameters, but further studies are needed to confirm it. |
| Databáze: | OpenAIRE |
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