Dually optimized polycaprolactone/collagen I microfiber scaffolds with stem cell capture and differentiation-inducing abilities promote bone regeneration
| Autor: | Zhengye Zhang, Guanghua Chen, Zecheng Li, Ye Ji, Jinglong Yan, Anlong Jiang, Hui Chi, Xiaoyan Wang, Jiaxin Li, Chengchao Song, Ravi Kumar Prajapati, Ang Li |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Scaffold Bone Regeneration business.product_category Polyesters Biomedical Engineering Bone Marrow Cells 02 engineering and technology 010402 general chemistry 01 natural sciences Regenerative medicine Collagen Type I Rats Sprague-Dawley chemistry.chemical_compound Tissue engineering Osteogenesis Apatites Microfiber Cell Adhesion Animals General Materials Science Cell adhesion Bone regeneration Cell Proliferation Tissue Scaffolds Stem Cells Bone Marrow Stem Cell Cell Differentiation General Chemistry General Medicine 021001 nanoscience & nanotechnology Rats 0104 chemical sciences Gene Expression Regulation chemistry Polycaprolactone Microscopy Electron Scanning Biophysics 0210 nano-technology business |
| Zdroj: | Journal of Materials Chemistry B. 7:7052-7064 |
| ISSN: | 2050-7518 2050-750X |
| DOI: | 10.1039/c9tb01359h |
| Popis: | Micro-nano based fibrous scaffolds have been extensively studied in regenerative medicine. Bone marrow stem cells (BMSCs) and BMP2-derived peptides, two other important components for tissue engineering, have been successfully used for bone regeneration. However, a scaffold that specifically captures BMSCs and delivers BMP2-derived peptides to promote osteogenic differentiation of enriched BMSCs has not been reported. In this study, a microfiber scaffold was constructed by coaxial electrospinning technology using a polyvinylpyrrolidone/bovine serum albumin/BMP2-derived peptide compound as the core solution and a polycaprolactone/collagen I compound as the shell solution. The scaffolds were further functionalized by covalent grafting of a BMSC affinity peptide (E7) to develop a dual drug release system for the delivery of the BMP2-derived peptide and E7. Structural analysis indicated that the microfibers had a uniform diameter and homogeneous core-shell structure. Fourier transform infrared spectroscopy (FTIR) revealed that E7 was covalently bonded onto the surface of the fibers. In vitro, the E7-modified scaffolds promoted the initial adhesion of BMSCs and were more favorable for BMSC survival. Furthermore, the BMP2-derived peptide loaded in the E7-modified scaffolds was released in a sustained manner and retained bioactivity, significantly improving the osteogenic differentiation of BMSCs. In vivo, scaffolds loaded with the BMP2-derived peptide and E7 (PCME scaffolds) led to enhanced new bone formation and defect closure in a rat calvarial defect model. Overall, the PCME scaffold simultaneously facilitated all three of the essential elements needed for bone tissue engineering, providing a promising method for bone regeneration. |
| Databáze: | OpenAIRE |
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