Ciglitazone induces caspase-independent apoptosis via p38-dependent AIF nuclear translocation in renal epithelial cells
| Autor: | Yong Keun Kim, Chang Soo Yoon, Chae Hwa Kwon |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
MAPK/ERK pathway
medicine.medical_specialty Programmed cell death Cell Survival Pyridines Blotting Western Active Transport Cell Nucleus Fluorescent Antibody Technique Apoptosis Toxicology p38 Mitogen-Activated Protein Kinases Cell Line Clofibric Acid Troglitazone Internal medicine Ciglitazone medicine Animals Chromans Caspase Cell Nucleus Membrane Potential Mitochondrial Dose-Response Relationship Drug biology Caspase-Independent Apoptosis MEK inhibitor Fibric Acids G1 Phase Imidazoles Apoptosis Inducing Factor Epithelial Cells Opossums Flow Cytometry Cell biology PPAR gamma Kidney Tubules Endocrinology biology.protein Apoptosis-inducing factor Thiazolidinediones |
| Zdroj: | Toxicology. 244:13-24 |
| ISSN: | 0300-483X |
| DOI: | 10.1016/j.tox.2007.10.019 |
| Popis: | Peroxisome proliferator-activated receptor gamma (PPARgamma) agonists have been reported to induce apoptosis in a variety of cell types including renal proximal epithelial cells. However, the underlying mechanism of cell death induced by PPARgamma agonists has not been clearly defined in renal proximal tubular cells. This study was therefore undertaken to determine the mechanism by which ciglitazone, a synthetic PPARgamma agonist, induces apoptosis in opossum kidney (OK) cells, an established renal epithelial cell line. Ciglitazone treatment induced apoptotic cell death in a dose- and time-dependent manner. Ciglitazone caused a transient activation of ERK and sustained activation of p38 MAP kinase. Ciglitazone-mediated cell death was attenuated by the p38 inhibitor SB203580 and transfection of dominant-negative form of p38, but not by the MEK inhibitor U0126, indicating that p38 MAP kinase activation is involved in the ciglitazone-induced cell death. Although ciglitazone-induced caspase-3 activation, the ciglitazone-mediated cell death was not affected by the caspase-3 inhibitor DEVD-CHO. Ciglitazone-induced mitochondrial membrane depolarization and apoptosis-inducing factor (AIF) nuclear translocation and these effects were prevented by the p38 inhibitor. These results suggest that ciglitazone induces caspase-independent apoptosis through p38 MAP kinase-dependent AIF nuclear translocation in OK renal epithelial cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect