Protocellular CRISPR/Cas‐Based Diffusive Communication Using Transcriptional RNA Signaling

Autor:	Shuo Yang, Alex Joesaar, Bas W. A. Bögels, Stephen Mann, Tom F. A. de Greef
Přispěvatelé:	Synthetic Biology, Chemical Biology, Computational Biology, ICMS Core
Rok vydání:	2022
Předmět:	Synthetic Protocells Communication CRISPR-Cas Systems/genetics DNA RNA/genetics General Medicine General Chemistry Catalysis Enzymes RNA Molecular Communication Artificial Cells CRISPR-Cas Systems Biophysical Chemistry
Zdroj:	Angewandte Chemie. International Edition, 61, 26, pp. 1-9 Angewandte Chemie. International Edition, 61, 1-9 Angewandte Chemie-International Edition, 61(26):e202202436. Wiley Angewandte Chemie (International Edition), 61(26)
ISSN:	1521-3773 1433-7851
Popis:	Protocells containing enzyme-driven biomolecular circuits that can process and exchange information offer a promising approach for mimicking cellular features and developing molecular information platforms. Here, we employ synthetic transcriptional circuits together with CRISPR/Cas-based DNA processing inside semipermeable protein-polymer microcompartments. We first establish a transcriptional protocell that can be activated by external DNA strands and produce functional RNA aptamers. Subsequently, we engineer a transcriptional module to generate RNA strands functioning as diffusive signals that can be sensed by neighboring protocells and trigger the activation of internalized DNA probes or localization of Cas nucleases. Our results highlight the opportunities to combine CRISPR/Cas machinery and DNA nanotechnology for protocellular communication and provide a step towards the development of protocells capable of distributed molecular information processing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b340951cd355bd9216b49e3b3c6b4a3 https://doi.org/10.1002/anie.202202436 Zobrazit plný text záznamu Plný text