Antiviral effect of silymarin against Zika virus in vitro
| Autor: | Letícia Trindade Almeida, Camila Carla da Silva Caetano, Rafaela Lameira Souza Lima, Kamila Lorene Soares Rocha, Danilo Bretas de Oliveira, José Carlos de Magalhães, Tales Fernando da Silva, Breno de Mello Silva, Ariane Coelho Ferraz, Ana Cláudia dos Santos Pereira Andrade, Cintia Lopes de Brito Magalhães, Fernanda Caetano Camini |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Antioxidant Veterinary (miscellaneous) medicine.medical_treatment 030231 tropical medicine Pharmacology Virus Replication Antiviral Agents Antioxidants Zika virus law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law medicine Animals Humans Viability assay EC50 biology Dose-Response Relationship Drug business.industry Ribavirin Zika Virus 030108 mycology & parasitology biology.organism_classification In vitro Infectious Diseases chemistry Insect Science Parasitology business Phytotherapy Viral load Silymarin |
| Zdroj: | Acta tropica. 211 |
| ISSN: | 1873-6254 |
| Popis: | Zika virus (ZIKV) epidemic and its association with severe neurological syndromes have raised worldwide concern. Despite the great clinical relevance of this infection, no vaccine or specific treatment is available and the search for antiviral compounds against ZIKV is extremely necessary. Several natural compounds, such as silymarin, exhibit antioxidant, hepatoprotective, and antiviral properties; however, the antiviral potential of this compound remains partially investigated. Therefore, the objective of this study was to evaluate in vitro the antiviral activity of silymarin against ZIKV infection. Global antiviral activity, dose-dependent, plaque reduction, and time-of-drug-addition assays were used to determine the anti-ZIKV activity of silymarin. Additionally, to start characterizing the mechanisms of action we determined whether silymarin could have a virucidal effect and inhibit viral adsorption and penetration stages. Regarding its global antiviral activity, silymarin showed significant inhibition of ZIKV infection, protecting cells infected with EC50 equal to 34.17μg/mL, with a selectivity index greater than 17 and 4x greater than that of the positive control (ribavirin). Its greatest efficiency was achieved at 125μg/mL, whose cell viability did not differ from the control without infection and treatment. Furthermore, treatment with silymarin reduced viral load by up to two logs (> 90%) concerning viral control, when evaluating virucidal activity and the precocious times of infection. Thus, our results set to show the promising anti-ZIKV activity of silymarin, which does not seem to have a single inhibition mechanism, acting at different times of infection, and still has the advantage of silymarin be a phytotherapy already available on the market. |
| Databáze: | OpenAIRE |
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