Functional interactions between the Epstein-Barr virus BZLF1 protein and the promyelocytic leukemia protein
Autor: | Brandy L. Bowling, Amy L. Adamson |
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Rok vydání: | 2005 |
Předmět: |
Transcriptional Activation
Cancer Research viruses Blotting Western Gene Expression Plasma protein binding Promyelocytic Leukemia Protein medicine.disease_cause Chromosomes Transactivation Promyelocytic leukemia protein Viral Proteins Interferon Genes Reporter hemic and lymphatic diseases Virology MHC class I medicine Humans Luciferases Cellular localization Tumor Necrosis Factor alpha-Induced Protein 3 Microscopy Confocal biology Tumor Suppressor Proteins Histocompatibility Antigens Class I Intracellular Signaling Peptides and Proteins virus diseases Nuclear Proteins Proteins Epstein–Barr virus BZLF1 Cell biology Artificial Gene Fusion Neoplasm Proteins DNA-Binding Proteins Infectious Diseases Microscopy Fluorescence biology.protein Cancer research Trans-Activators medicine.drug HeLa Cells Protein Binding Transcription Factors |
Zdroj: | Virus research. 117(2) |
ISSN: | 0168-1702 |
Popis: | The Epstein-Barr virus immediate-early protein BZLF1 (Z) has been shown to alter the cellular localization of the promyelocytic leukemia (PML) protein. PML has important implications for growth control, apoptosis, anti-viral effects and many more processes. Here we further examined the relationship between PML and the Epstein-Barr virus Z protein. We examined the effect of Z expression on PML protein levels, and the effect of increased PML protein levels on Z-mediated dispersion of PML bodies. We found that increased levels of PML protein, such as through interferon treatment, were able to suppress Z-mediated PML body dispersion. We also studied the consequences of PML dispersion by Z, by examining p21 transactivation, A20 transactivation, and MHC Class I presentation levels in Z-expressing cells. We found that, while Z-mediated dispersion of PML did not affect MHC Class I presentation, it did alter p21 and A20 expression. In addition, we found that increased levels of PML were able to prevent Z protein binding to mitotic chromosomes. Our work implies that the balance of PML and Z levels in cells may affect how each protein functions. |
Databáze: | OpenAIRE |
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