Bacteriophage λ N protein inhibits transcription slippage by Escherichia coli RNA polymerase
| Autor: | Carolyn Court, Donald L. Court, Lucyna Lubkowska, Ding J. Jin, Mikhail Kashlev, Adam R. Parks |
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| Rok vydání: | 2014 |
| Předmět: |
Base Sequence
Transcription Genetic General transcription factor Nucleic Acid Enzymes Escherichia coli Proteins Termination factor RNA-dependent RNA polymerase DNA-Directed RNA Polymerases Biology beta-Galactosidase Bacteriophage lambda Molecular biology chemistry.chemical_compound Terminator (genetics) chemistry Genes Reporter Transcription (biology) Antitermination RNA polymerase Escherichia coli Genetics biology.protein Viral Regulatory and Accessory Proteins Polymerase |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gku203 |
| Popis: | Transcriptional slippage is a class of error in which ribonucleic acid (RNA) polymerase incorporates nucleotides out of register, with respect to the deoxyribonucleic acid (DNA) template. This phenomenon is involved in gene regulation mechanisms and in the development of diverse diseases. The bacteriophage λ N protein reduces transcriptional slippage within actively growing cells and in vitro. N appears to stabilize the RNA/DNA hybrid, particularly at the 5′ end, preventing loss of register between transcript and template. This report provides the first evidence of a protein that directly influences transcriptional slippage, and provides a clue about the molecular mechanism of transcription termination and N-mediated antitermination. |
| Databáze: | OpenAIRE |
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