Proteomic Signature of Nucleus Pulposus in Fetal Intervertebral Disc
| Autor: | Rishi Mugesh Kanna, Chitraa Tangavel, Raveendran Muthurajan, Monica Steffi Matchado, Ajoy Prasad Shetty, Dilip Chand Raja Soundararajan, Shanmuganathan Rajasekaran, Kuppamuthu Dharmalingam, Sharon Miracle Nayagam, K. S. Sri Vijay Anand |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Medicine
03 medical and health sciences Ribosomal protein Thrombospondin 1 Protein purification medicine Orthopedics and Sports Medicine low back pain 030304 developmental biology 0303 health sciences Thrombospondin biology intervertebral disc degeneration business.industry Biglycan 030302 biochemistry & molecular biology lcsh:R Intervertebral disc Basic Study Cell biology medicine.anatomical_structure Histone fetal intervertebral disc proteome Proteome biology.protein disc degeneration Surgery business |
| Zdroj: | Asian Spine Journal, Vol 14, Iss 4, Pp 409-420 (2020) Asian Spine Journal |
| ISSN: | 1976-7846 1976-1902 |
| Popis: | Study Design: Profiling proteins expressed in the nucleus pulposus of fetal intervertebral disc (IVD).Purpose: To evaluate the molecular complexity of fetal IVDs not exposed to mechanical, traumatic, inflammatory, or infective insults to generate improved knowledge on disc homeostasis.Overview of Literature: Low back pain is the most common musculoskeletal disorder, causing a significant reduction in the quality of life, and degenerative disc disorders mainly contribute to the increasing socioeconomic burden. Despite extensive research, the causative pathomechanisms behind degenerative disc disorders are poorly understood. Precise molecular studies on the intricate biological processes involved in maintaining normal disc homeostasis are needed.Methods: IVDs of nine fetal specimens obtained from medical abortions were used to dissect out the annulus fibrosus and nucleus pulposus under sterile operating conditions. Dissected tissues were transferred to sterile Cryovials and snap frozen in liquid nitrogen before transporting to the research laboratory for protein extraction and further liquid chromatography tandem mass spectrometry (LC-MS/ MS) analysis. Collected data were further analyzed using Gene Functional Classification Tool in DAVID and STRING databases.Results: A total of 1,316 proteins were identified through LC-MS/MS analysis of nine fetal IVD tissues. Approximately 247 proteins present in at least four fetal discs were subjected to further bioinformatic analysis. The following 10 clusters of proteins were identified: collagens, ribosomal proteins, small leucine-rich proteins, matrilin and thrombospondin, annexins, protein disulfide isomerase family proteins and peroxiredoxins, tubulins, histones, hemoglobin, and prolyl 4-hydroxylase family proteins.Conclusions: This study provides fundamental information on the proteome networks involved in the growth and development of healthy fetal discs in humans. Systematic cataloging of proteins involved in various structural and regulatory processes has been performed. Proteins expressed most abundantly (collagen type XIV alpha 1 chain, biglycan, matrilin 1, and thrombospondin 1) in their respective clusters also elucidate the possibility of utilizing these proteins for potential regenerative therapies. |
| Databáze: | OpenAIRE |
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