Identification of the intermediate host of Gongylonema sp., the etiological agent of the necrotic oropharyngeal disease of the Scops owl (Otus scops)
| Autor: | Raúl Alonso, D. Gil-Tapetado, D. Bravo-Barriga, Fernando Esperón, F.J. Cabrero-Sañudo, Francisca Lopes, E. Frontera, Patricia Orejas |
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| Přispěvatelé: | Morris Animal Foundation, Esperón, F [0000-0002-8810-5071], Bravo-Barriga, D [0000-0003-3481-7310], Frontera, E [0000-0003-0222-7850], Cabrero-Sañudo, F J [0000-0001-8477-3593], Gil-Tapetado, D [0000-0002-2147-4040], Orejas, P [0000-0003-1637-2557], Esperón, F, Bravo-Barriga, D, Frontera, E, Cabrero-Sañudo, F J, Gil-Tapetado, D, Orejas, P |
| Rok vydání: | 2021 |
| Předmět: |
Male
Intermediate host Oriental cockroaches Necrotic oropharyngeal disease Zoology Oropharynx Cockroaches Spirurida Infections Gongylonema sp Otus scops Host-Parasite Interactions Blattodea parasitic diseases Parasite hosting Animals Spirurida Gongylonema biology Bird Diseases Scops owl Pharyngeal Diseases B. orientalis biology.organism_classification Strigiformes Infectious Diseases Nematode Spain Parasitology Spiruroidea |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 5 Pág. Since 1997, fledgling Scops owls (Chordata: Strigidae) have been brought to the Brinzal Owl Rescue Centre (Madrid, Spain) with severe lesions in their oral cavities. Lesions consist of the presence of proliferative necrotic material in the oral cavity resulting in white plaques, which can lead to death by starvation. This disease has been detected in owls only within the limits of the city of Madrid. The etiologic agent has been identified as Gongylonema sp. (Nematoda: Spirurida), a nematode genus that includes a coprophagous arthropod as intermediate host in its cycle. The aim of this study was to identify the intermediate host of the parasite. Our work was structured in four component phases: i) Diet study of newborn chicks; ii) trapping arthropods that could be intermediate hosts; iii) molecular detection of the parasite in the selected arthropods: and iv) molecular characterization of the detected parasites by amplifying the cox1 gene. Four male owls were radio-tagged in order to locate their nests and a camera trap was placed to identify the prey brought to the owlets. Secondly, the arthropods present in the hunting areas of the owls were sampled, identified and analyzed by real time PCR (rtPCR). Only oriental cockroaches, B. orientalis (Arthropoda: Blattodea), were positive by rtPCR detection of Gongylonema sp. (66.7%). The nematodes obtained from cockroaches had a 99.8% identity of the cox1 gene with the Gongylonema sp. isolated for the first time in a Scops owl. Furthermore, these sequences only showed an This work was supported by the Morris Animal Foundation (grant number D14ZO-834). |
| Databáze: | OpenAIRE |
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