Determination of the hepatocellularity number for human, dog, rabbit, rat and mouse livers from protein concentration measurements
| Autor: | Anna-Karin Sohlenius-Sternbeck |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male medicine.medical_specialty Liver cytology Cell Count Biology Toxicology Cryopreservation Rats Sprague-Dawley Mice chemistry.chemical_compound Dogs Species Specificity In vivo Internal medicine medicine Animals Humans Aged Proteins Rabbit rat DNA General Medicine Middle Aged biology.organism_classification Molecular biology In vitro Rats EGTA Endocrinology medicine.anatomical_structure Liver chemistry Hepatocyte Hepatocytes Female Rabbits Xenobiotic |
| Zdroj: | Toxicology in Vitro. 20:1582-1586 |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2006.06.003 |
| Popis: | Biologically based scaling factors have to be used to predict in vivo metabolic clearance of xenobiotics from data obtained in vitro. Although standard values for the hepatocellularity numbers for different species are used in the literature, detailed information on the determination of these values has only been presented for humans and rats, and somewhat different results have been obtained in different studies. The present work was undertaken in order to determine the number of hepatocytes per gram of liver for human, dog, rabbit, rat and mouse livers. Hepatocellularity numbers were calculated from the ratio between the liver protein concentration and the protein concentration in the corresponding hepatocyte suspension. For human, rabbit, rat and mouse livers, the hepatocellular values were in the same range, more precisely 139+/-25, 114+/-20, 117+/-30 and 135+/-10 million cells per gram of liver, respectively. However, for the dog liver, the corresponding value was as high as 215+/-45 million cells per gram. These values should be of importance during the scaling process of intrinsic clearance for xenobiotics in hepatocytes to in vivo hepatic clearance. |
| Databáze: | OpenAIRE |
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