A review on recent advances in the enrichment of glycopeptides and glycoproteins by liquid chromatographic methods: 2016–Present
| Autor: | Kishore K.R. Tetala, Mona Kumari |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Glycan Glycosylation Chromatography biology Clinical Biochemistry Glycopeptides Mass spectrometry Ligand (biochemistry) Biochemistry Cellular signal transduction Glycopeptide Analytical Chemistry chemistry.chemical_compound Enzyme chemistry Polysaccharides biology.protein Glycoprotein Protein Processing Post-Translational Chromatography Liquid Glycoproteins |
| Zdroj: | ELECTROPHORESIS. 43:388-402 |
| ISSN: | 1522-2683 0173-0835 |
| Popis: | Among various protein post-translational modifications (PTMs), glycosylation has received special attention due to its immense role in molecular interactions, cellular signal transduction, immune response etc. Aberration in glycan moieties of a glycoprotein is associated with cancer, diabetes, and bacterial and viral infections. In biofluids (plasma, saliva, urine, milk etc.), glycoproteins are low in abundance and are masked by the presence of high abundant proteins. Hence, prior to their identification using mass spectrometry (MS) methods, liquid chromatography (LC) based approaches were widely used. A general enrichment strategy involves a protein digestion step, followed by LC based enrichment and desorption of glycopeptides, and enzymatic excision of the glycans. The focus of this review paper is to highlight the articles published since 2016 that dealt with different LC based approaches for glycopeptide and glycoprotein enrichment. The preparation of stationary phases, their surface activation, and ligand immobilization strategies have been discussed in detail. Finally, the major developments and future trends in the field have been summarized. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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