Psychrilyobacter atlanticus gen. nov., sp. nov., a marine member of the phylum Fusobacteria that produces H2 and degrades nitramine explosives under low temperature conditions
| Autor: | Jalal Hawari, Jian-Shen Zhao, Dominic Manno |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Canada
Geologic Sediments Sequence analysis Stereochemistry Molecular Sequence Data Microbiology Fusobacteria Explosive Agents Species Specificity Phylogenetics 23S ribosomal RNA RNA Ribosomal 16S Atlantic Ocean Nitrobenzenes Phylogeny Ecology Evolution Behavior and Systematics Base Composition Aniline Compounds biology Ecology Genes rRNA Sequence Analysis DNA General Medicine Ribosomal RNA biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques Cold Temperature RNA Ribosomal 23S Type species Phenotype Fermentation Bacteria Hydrogen |
| Zdroj: | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 59:491-497 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.65263-0 |
| Popis: | A Gram-negative and obligately anaerobic marine bacterium, strain HAW-EB21(T), was isolated in a previous study from marine sediment from the Atlantic Ocean, near Halifax Harbor, Canada, and found to have the potential to degrade both hexahydro-1,3,5-trinitro-1,3,5-triazine and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine. In the present study, phylogenetic analyses showed that strain HAW-EB21(T) was only distantly related to the genera Propionigenium and Ilyobacter with 6.6-7.5 % and 8.2-10.5 % dissimilarity as measured by 16S rRNA and 23S rRNA gene sequence analyses, respectively. Strain HAW-EB21(T) displayed unique properties in being psychrotrophic (18.5 degrees C optimum) and unable to utilize any of the carbon substrates (succinate, l-tartrate, 3-hydroxybutyrate, quinate or shikimate) used for isolating members of the genera Propionigenium and Ilyobacter. Strain HAW-EB21(T) utilized glucose, fructose, maltose, N-acetyl-d-glucosamine, citrate, pyruvate, fumarate and Casitone as carbon sources and produced H(2) and acetate as the major fermentation products. Cells grown at 10 degrees C produced C(15 : 1) (30 %), C(16 : 1)omega7 (15 %) and C(16 : 0) (16 %) as major membrane fatty acids. The novel strain had a genomic DNA G+C content of 28.1 mol%, lower than the values of the genera Ilyobacter and Propionigenium. Based on the present results, the novel isolate is suggested to be a member of a new genus for which the name Psychrilyobacter atlanticus gen. nov., sp. nov. is proposed. The type strain of the type species is HAW-EB21(T) (=DSM 19335(T)=JCM 14977(T)). |
| Databáze: | OpenAIRE |
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