Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow
| Autor: | Stefanie Hahne, Katrin Lehmann, Claudia Haftmann, Jannis Kummer, Melanie Weber, Silvia Kühnel, Daniel Schulz, Stefan Kröger, Ralf Köhler, Richard K. Addo, Jakob Zimmermann, Ulrike Menzel, Frederik Heinrich, Andreas Radbruch, Patrick Maschmeyer, Rebecca Cornelis, Anja E. Hauser, Sai T. Reddy, Francesco Siracusa, Gitta Anne Heinz, Ulrik Stervbo, Özen Sercan-Alp, Mir-Farzin Mashreghi, Jonathan Stefanowski, Pawel Durek, René Riedel, Hyun-Dong Chang, Victor Greiff, Marta Ferreira-Gomes, Cora Klaeden, Mairi McGrath, Kerstin Westendorf, Sandra Naundorf |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
General Physics and Astronomy Immunological memory Transcriptome 0302 clinical medicine 610 Medicine & health lcsh:Science education.field_of_study B-Lymphocytes Multidisciplinary Cell Cycle RNA sequencing Isotype medicine.anatomical_structure Antibody Stromal cell Science B-cell receptor Population Receptors Antigen B-Cell Vascular Cell Adhesion Molecule-1 Spleen Animals Wild Bone Marrow Cells Mice Transgenic Biology General Biochemistry Genetics and Molecular Biology Article Antibodies 03 medical and health sciences Antigen Adjuvants Immunologic medicine Animals ddc:610 education B cell Cell Proliferation B cells General Chemistry Immunoglobulin Class Switching B-1 cell Mice Inbred C57BL 030104 developmental biology Gene Expression Regulation Immunology biology.protein lcsh:Q Bone marrow Stromal Cells 610 Medizin und Gesundheit Immunologic Memory 030215 immunology |
| Zdroj: | Nature Communications Riedel, René; Addo, Richard; Ferreira-Gomes, Marta; Heinz, Gitta Anne; Heinrich, Frederik; Kummer, Jannis; Greiff, Victor; Schulz, Daniel; Klaeden, Cora; Cornelis, Rebecca; Menzel, Ulrike; Kröger, Stefan; Stervbo, Ulrik; Köhler, Ralf; Haftmann, Claudia; Kühnel, Silvia; Lehmann, Katrin; Maschmeyer, Patrick; McGrath, Mairi; Naundorf, Sandra; ... (2020). Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow. Nature Communications, 11(1), p. 2570. Springer Nature 10.1038/s41467-020-16464-6 Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020) Nature Communications, 11 (1) bioRxiv |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-16464-6 |
| Popis: | At present, it is not clear how memory B lymphocytes are maintained over time, and whether only as circulating cells or also residing in particular tissues. Here we describe distinct populations of isotype-switched memory B lymphocytes (Bsm) of murine spleen and bone marrow, identified according to individual transcriptional signature and B cell receptor repertoire. A population of marginal zone-like cells is located exclusively in the spleen, while a population of quiescent Bsm is found only in the bone marrow. Three further resident populations, present in spleen and bone marrow, represent transitional and follicular B cells and B1 cells, respectively. A population representing 10-20% of spleen and bone marrow memory B cells is the only one qualifying as circulating. In the bone marrow, all cells individually dock onto VCAM1+ stromal cells and, reminiscent of resident memory T and plasma cells, are void of activation, proliferation and mobility. Nature Communications, 11 (1) ISSN:2041-1723 |
| Databáze: | OpenAIRE |
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