Liquid nitrogen storage of cultured T lymphocytes

Autor:	J. L. Mckimm-Breschkin, Jacques F. A. P. Miller, P.L. Mottram
Rok vydání:	1985
Předmět:	Chromatography Chemistry Cell Survival T cell T-Lymphocytes Immunology Cell Preservation Biological T lymphocyte Liquid nitrogen medicine.anatomical_structure Biochemistry Cell culture Freezing medicine Immunology and Allergy Humans
Zdroj:	Journal of immunological methods. 78(2)
ISSN:	0022-1759
Popis:	The condition required for storing and recovering IL-2-dependent T cell clones from liquid nitrogen were investigated. For maximum cell recovery, cultured T lymphocytes were precooled at 4°C for 15 min in medium containing 10% DMSO and 20% FCS before storage in liquid nitrogen. This method allowed adequate time for DMSO to penetrate the cells before freezing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b1a9e6470f11a3e066004e05502ce2a https://pubmed.ncbi.nlm.nih.gov/3872914 Zobrazit plný text záznamu