Polymorphism in the immunoglobulin VH gene V1-69 affects susceptibility to rheumatoid arthritis in subjects lacking the HLA-DRB1 shared epitope
| Autor: | E. Zd’árský, Ali H. Hajeer, Ravinder N. Maini, Z. Cimburek, Rizgar A. Mageed, S Ruzickova, S P Moyes, W. E. R. Ollier, Jiří Vencovský |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Genotype Population Immunoglobulin Variable Region Human leukocyte antigen Immunogenetics Biology Genetic determinism Arthritis Rheumatoid Epitopes Rheumatology Genetic predisposition Humans Pharmacology (medical) Genetic Predisposition to Disease education HLA-DRB1 Gene Alleles Aged Aged 80 and over education.field_of_study Polymorphism Genetic Genes Immunoglobulin HLA-DR Antigens Middle Aged Molecular biology Immunology Female Restriction fragment length polymorphism Immunoglobulin Heavy Chains HLA-DRB1 Chains |
| Zdroj: | Rheumatology (Oxford, England). 41(4) |
| ISSN: | 1462-0324 |
| Popis: | OBJECTIVE To investigate the contribution of polymorphism in the immunoglobulin heavy chain variable region V1-69 gene set to genetic susceptibility to rheumatoid arthritis (RA) in Czech and British patients. METHODS We used V1-69 gene sequence-specific polymerase chain reaction (PCR) and restriction enzyme digestion to study polymorphism in the V1-69 gene set in germline DNA of 109 Czech and 159 British RA patients and 164 ethnically matched controls. Polymorphism was further studied by nucleotide sequencing of the V1-69 gene locus in germline DNA. RESULTS We found that all patients and controls had at least one V1-69 gene copy. In the Czech RA cohort, the dimorphic nucleotide in codon 73 of V1-69 (GAA or AAA) was present in the homozygous form 73(A/A) in 31 of 109 (28.4%) RA patients vs 12 of 79 (15.2%) controls [odds ratio (OR)=2.22, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|