Silencing D. melanogaster lgr1 impairs transition from larval to pupal stage
| Autor: | Matthias B. Van Hiel, Jozef Vanden Broeck, Hans Peter Vandersmissen, Rut Vleugels, Tom Van Loy |
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| Rok vydání: | 2014 |
| Předmět: |
Male
animal structures Biology Halloween genes Receptors G-Protein-Coupled Animals Genetically Modified chemistry.chemical_compound Endocrinology RNA interference Melanogaster Animals Drosophila Proteins Gene Silencing Phylogeny Genetics Ecdysteroid Gene knockdown Gene Expression Profiling fungi GTP-Binding Protein beta Subunits Pupa Gene Expression Regulation Developmental biology.organism_classification GTP-Binding Protein alpha Subunits White (mutation) Drosophila melanogaster chemistry Larva Animal Science and Zoology Female Pupariation |
| Zdroj: | General and comparative endocrinology. 209 |
| ISSN: | 1095-6840 |
| Popis: | G protein-coupled receptors (GPCRs) play key roles in a wide diversity of physiological processes and signalling pathways. The leucine-rich repeats containing GPCRs (LGRs) are a subfamily that is well-conserved throughout most metazoan phyla and have important regulatory roles in vertebrates. Here, we report on the critical role of Drosophila melanogaster LGR1, the fruit fly homologue of the vertebrate glycoprotein hormone receptors, in development as a factor involved in the regulation of pupariation. Transcript profiling revealed that lgr1 transcripts are most abundant in third instar larvae and adult flies. The tissues displaying the highest transcript levels were the hindgut, the rectum and the salivary glands. Knockdown using RNA interference (RNAi) demonstrated that white pupa formation was severely suppressed in D. melanogaster lgr1 RNAi larvae. Associated with this developmental defect was a reduced ecdysteroid titer, which is in line with significantly reduced transcript levels detected for the Halloween genes shadow (sad) and spookier (spok) in the third instar lgr1 RNAi larvae compared to the control condition. publisher: Elsevier articletitle: Silencing D. melanogaster lgr1 impairs transition from larval to pupal stage journaltitle: General and Comparative Endocrinology articlelink: http://dx.doi.org/10.1016/j.ygcen.2014.08.006 content_type: article copyright: Copyright © 2014 Elsevier Inc. All rights reserved. ispartof: General and Comparative Endocrinology vol:209 pages:135-147 ispartof: location:United States status: published |
| Databáze: | OpenAIRE |
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