The effects of antioxidants on a porcine model of liver hemorrhage
| Autor: | Agathi Kondi-Pafiti, Irene P. Stergiou, Cleanthi Dimas, Apostolos Papalois, Evangelos Lolis, Iosifina Karmaniolou, Vasileios Smyrniotis, Anastasios I. Mylonas, Nikolaos Arkadopoulos, Nikolaos F. Orfanos |
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| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty Resuscitation Swine medicine.medical_treatment 030232 urology & nephrology Apoptosis Deferoxamine Shock Hemorrhagic Critical Care and Intensive Care Medicine 030226 pharmacology & pharmacy Gastroenterology Antioxidants Acetylcysteine Random Allocation 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals Hepatectomy Random allocation business.industry Crystalloid Solutions Immunohistochemistry Disease Models Animal Liver Shock (circulatory) Hemorrhagic shock Surgery Liver hemorrhage Isotonic Solutions medicine.symptom business medicine.drug |
| Zdroj: | Journal of Trauma and Acute Care Surgery. 80:964-971 |
| ISSN: | 2163-0755 |
| DOI: | 10.1097/ta.0000000000001026 |
| Popis: | The aim of this study is to assess the efficacy of the combination of N-acetylcysteine (NAC) and deferoxamine (DFO) in the resuscitation from hemorrhagic shock in a porcine model of bleeding during hepatectomy.Twenty-one pigs were divided randomly to three groups: Sham (S) group, n = 5; fluid (F) resuscitation group, n = 8; and fluid plus NAC plus DFO (NACDFO) resuscitation group, n = 8. The animals of groups F and NACDFO were subjected to left hepatectomy and controlled hemorrhage from the traumatic liver surface. Shock was established within 10 minutes and maintained for 30 minutes at mean arterial pressure (MAP) of 30 to 40 mm Hg. Resuscitation followed the shock period with crystalloids and colloids. Group NACDFO received additionally NAC and DFO in doses of 200 mg/kg and 65 mg/kg, respectively. The total time of the experiment was 6 hours.Animal weight, blood loss, excised liver mass, and MAP at the end of the shock period were comparable between experimental groups. Group NACDFO received significantly lower volume of both crystalloids and colloids (35% and 42% less, respectively) compared to group F. Hepatocellular proliferation (proliferating cell nuclear antigen) was higher in the antioxidant group. Apoptosis, measured by caspase-3, was restored to sham group levels when NAC and DFO were administered.Our experimental study showed that coadministration of NAC and DFO during liver hemorrhage can decrease the amounts of fluids needed for resuscitation. Moreover, the antioxidant combination restores the energy dependent apoptosis and proliferation of the hepatocytes. |
| Databáze: | OpenAIRE |
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