SCHEEPDOG: programming electric cues to dynamically herd large-scale cell migration
| Autor: | Gawoon Shim, Alejandro Rossello-Martinez, Linus Wang, Daniel J. Cohen, Tom J. Zajdel |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Scheme (programming language)
Cell type Collective behavior Histology Computer science Distributed computing Transduction (psychology) Article Pathology and Forensic Medicine Collective migration 03 medical and health sciences 0302 clinical medicine Cell Movement medicine Humans 030304 developmental biology computer.programming_language 0303 health sciences Scale (chemistry) Cancer Cell migration Cell Biology medicine.disease Computer control computer 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Cell Syst |
| Popis: | Directed cell migration is critical across biological processes spanning healing to cancer invasion, yet no existing tools allow real-time interactive guidance over such migration. We present a new bioreactor that harnesses electrotaxis—directed cell migration along electric field gradients—by integrating four independent electrodes under computer control to dynamically program electric field patterns, and hence steer cell migration. Using this platform, we programmed and characterized multiple precise, two-dimensional collective migration maneuvers in renal epithelia and primary skin keratinocyte ensembles. First, we demonstrated on-demand, 90-degree collective turning. Next, we developed a universal electrical stimulation scheme capable of programming arbitrary 2D migration maneuvers such as precise angular turns and migration in a complete circle. Our stimulation scheme proves that cells effectively time-average electric field cues, helping to elucidate the transduction time scales in electrotaxis. Together, this work represents an enabling platform for controlling cell migration with broad utility across many cell types. |
| Databáze: | OpenAIRE |
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