DNA/RNA markers for colorectal cancer risk in preserved stool specimens: a pilot study
| Autor: | Kawsar Z. Badsha, Jeffrey L. Ram, Marc D. Basson, Susan Land, Adhip P.N. Majumdar, Jordan M. Nechvatal, Adi L. Tarca, Ikuko Kato, Larry H. Matherly |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Cancer Research Colorectal cancer Bisulfite sequencing Molecular Sequence Data Estrogen receptor Pilot Projects Biology Risk Assessment Article 030218 nuclear medicine & medical imaging 03 medical and health sciences Feces 0302 clinical medicine Risk Factors medicine Biomarkers Tumor Humans RNA Neoplasm Aged medicine.diagnostic_test Base Sequence Stool test Estrogen Receptor alpha General Medicine Methylation DNA Neoplasm Sequence Analysis DNA DNA Methylation Middle Aged medicine.disease Molecular biology Reverse transcriptase ErbB Receptors Gene Expression Regulation Neoplastic Oncology CpG site Cyclooxygenase 2 030220 oncology & carcinogenesis DNA methylation Cancer research Feasibility Studies CpG Islands Female Colorectal Neoplasms |
| Popis: | Aims and Background Exfoliated cells in human stool offer excellent opportunities to non-invasively detect molecular markers associated with colorectal tumorigenesis, and to evaluate the effects of exposures to exogenous and endogenous carcinogenic or chemopreventive substances. This pilot study investigated the feasibility of determining DNA methylation and RNA expression simultaneously in stool specimens treated with a single type of nucleic acid preservatives. Methods Stool specimens from 56 volunteers that were preserved up to a week with RNAlater were used in this study. Bisulfite sequencing was used to determine methylation at 27 CpG loci on the estrogen receptor 1 ( ESR1) promoter. Taqman assay was used for quantitative reverse transcription polymerase chain reactions to measure cyclooxygenase 2 ( COX2) and epidermal growth factor receptor ( EGFR) mRNA expression. Subjects’ basic demographic and other selected risk factors for colorectal cancer were captured through questionnaires and correlated with the levels of these markers. Results Less than 10% of the samples failed in individual assays. Overall, 24.0% of the CpG loci on the ESR1 promoter were methylated. COX2 expression and alcohol use were positively correlated; an inverse association was present between EGFR expression and cigarette smoking; and subjects using anti-diabetic medication had higher ESR1 methylation. In addition, higher EGFR expression levels were marginally associated with history of polyps and family history of colorectal cancer. Conclusions The present study demonstrates that simultaneous analyses for DNA and RNA markers are feasible in stool samples treated with a single type of nucleotide preservatives. Among several associations observed, the association between EGFR expression and polyps deserves further investigation as a potential target for colorectal cancer screening. Larger studies are warranted to confirm some of our observations. |
| Databáze: | OpenAIRE |
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