Lumican mediates HTB94 chondrosarcoma cell growth via an IGF-IR/Erk1/2 axis
| Autor: | Eirini Maria Giatagana, George N. Tzanakakis, Aristidis Tsatsakis, Dragana Nikitovic, Aikaterini Berdiaki, Antonis Papoutsidakis, Demetrios A. Spandidos, Ioanna Spyridaki |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Lumican Decorin MAP Kinase Signaling System medicine.medical_treatment insulin-like growth factor receptor I Bone Neoplasms Biology Receptor IGF Type 1 Extracellular matrix 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Line Tumor medicine cell growth Humans Insulin-Like Growth Factor I RNA Small Interfering small leucine-rich proteoglycans Cell Proliferation chondrosarcoma Cell growth Growth factor Biglycan Articles Cell biology carbohydrates (lipids) 030104 developmental biology Oncology Cell culture 030220 oncology & carcinogenesis extracellular regulated kinase 1/2 |
| Zdroj: | International Journal of Oncology |
| ISSN: | 1791-2423 1019-6439 |
| Popis: | Chondrosarcoma is a malignant bone tumor characterized by the production of a modified cartilage‑type extracellular matrix (ECM). In the present study, the expression levels of the small leucine‑rich proteoglycans (SLRPs), decorin, biglycan and lumican, were examined in the HTB94 human chondrosarcoma cell line. HTB94 cells were found to express and secrete the 3 SLRP members. RT‑qPCR and western blot analysis demonstrated that lumican was the most abundantly secreted SLRP, whereas decorin and biglycan expression levels were low. The utilization of short interfering RNA specific for the decorin, biglycan, and lumican genes resulted in the efficient downregulation of the respective mRNA levels (P≤0.001). The growth of the HTB94 cells was stimulated by lumican (P≤0.001), whereas their migration and adhesion were not affected (P=NS). By contrast, these cellular functions were not sensitive to a decrease in low endogenous levels of decorin and biglycan. Lumicandeficiency significantly inhibited both basal and insulin‑like growth factor I (IGF‑I)‑induced HTB94 cell growth (P≤0.001 andP≤0.01, respectively). These effects were executed through the insulin‑like growth factor I receptor (IGF‑IR), whose activation was markedly attenuated (P≤0.01) in lumican‑deficient HTB94 cells. The downregulation of lumican induced the substantial inhibition of extracellular regulated kinase (ERK1/2) activation (P≤ 0.01), indicating that ERK1/2 is a necessary component of lumican/IGF‑IR‑mediated HTB94 cell proliferation. Moreover, the lumican‑deficient cells exhibit increased mRNA levels of p53 (P≤0.05), suggesting that lumican facilitates HTB94 cell growth through an IGF‑IR/ERK1/2/p53 signaling cascade. On the whole, the findings of the present study demonstrate that endogenous lumican is a novel regulator of HTB94 cell growth. |
| Databáze: | OpenAIRE |
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