Sustained expression of a neuron-specific isoform of the Taf1 gene in development stages and aging in mice
| Autor: | Satoshi Makino, Batmunkh Munkhbat, Jamiyansuren Jambaldorj, Gen Tamiya |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Gene isoform
Aging Neurogenesis Molecular Sequence Data Biophysics Biology Biochemistry Mice Open Reading Frames Transcription (biology) Cell Line Tumor Transcriptional regulation Animals Humans Protein Isoforms RNA Messenger Cloning Molecular Molecular Biology Transcription factor Gene Cell Proliferation Histone Acetyltransferases Neurons Regulation of gene expression Mice Inbred BALB C TATA-Binding Protein Associated Factors Messenger RNA Base Sequence Brain Gene Expression Regulation Developmental Exons Cell Biology Embryo Mammalian Molecular biology TAF1 Transcription Factor TFIID Head Cell Division |
| Zdroj: | Biochemical and Biophysical Research Communications. 425:273-277 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2012.07.081 |
| Popis: | TATA-box binding protein associated factor 1 (TAF1) protein is the largest and the essential component of the TFIID complex in the pathway of RNA polymerase II-mediated gene transcription, and it regulates transcription of a large number of genes related to cell division. The neuron-specific isoform of the TAF1 gene (N-TAF1), which we reported previously, may have an essential role in neurons through transcriptional regulation of many neuron-specific genes. In the present study, we cloned the full-length cDNA that encodes the mouse homologue of N-TAF1 (N-Taf1) protein. By carrying out of real time RT-PCR, we investigated the expression analysis of the N-Taf1 mRNA in mouse tissues and cell lines. As well as the human N-TAF1, the N-Taf1 showed limited expression in the brain and neuroblastoma, whereas Taf1 expressed elsewhere. Furthermore, in mouse embryo head or mouse brain, mRNA expression of TAF1 changes dramatically during development but N-Taf1 showed sustained expression. Our result suggests that the N-Taf1 gene has an important role in non-dividing neuronal cell rather than in cell division and proliferation during neurogenesis. |
| Databáze: | OpenAIRE |
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