Long Noncoding RNA CRNDE Promotes Multiple Myeloma Cell Growth by Suppressing miR-451
| Autor: | Yunfei Huang, Xin He, Dingjun Hao, Qining Wu, Yibin Meng, Yong-Cun Zhou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Cell cycle checkpoint Apoptosis Article miR-451 03 medical and health sciences 0302 clinical medicine RNA interference Cell Movement Genes Reporter Cell Line Tumor microRNA Humans Multiple myeloma (MM) Cell Proliferation Genetics Gene knockdown Cell growth Chemistry Cell Cycle Long noncoding RNAs (lncRNAs) General Medicine Cell cycle Long non-coding RNA Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Cell Transformation Neoplastic Oncology Tumor progression 030220 oncology & carcinogenesis Case-Control Studies Gene Knockdown Techniques Cancer research RNA Interference RNA Long Noncoding Multiple Myeloma Colorectal neoplasia differentially expressed (CRNDE) |
| Zdroj: | Oncology Research |
| ISSN: | 1555-3906 0965-0407 |
| Popis: | It has been determined that long noncoding RNAs (lncRNAs) are identified as a potential regulatory factor in multiple tumors as well as multiple myeloma (MM). However, the role of colorectal neoplasia differentially expressed (CRNDE) in the pathogenesis of MM remains unclear. In this study, we found that the CRNDE expression level, in MM samples and cell lines, is higher than that in the control detected by real-time qPCR, which is also closely related to tumor progression and poor survival in MM patients. Knockdown of CRNDE significantly inhibits the proliferative vitality of MM cells (U266 and RPMI-8226), induces cell cycle arrest in the G0/G1 phase, and promotes apoptosis. After being transfected with siRNA, miR-451 expression observably increases. Bioinformatics analysis and luciferase assay reveal the interaction by complementary bonding between CRNDE and miR-451. Pearsons correlation shows that CRNDE is negatively correlated to miR-451 expression in human MM samples. Subsequently, miR-451 inhibitor rescues the inhibited tumorigenesis induced by CRNDE knockdown. Our study illustrates that lncRNA CRNDE induces the proliferation and antiapoptosis capability of MM by acting as a ceRNA or molecular sponge via negatively targeting miR-451, which could act as a novel diagnostic marker and therapeutic target for MM. |
| Databáze: | OpenAIRE |
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