In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin

Autor: Eleftheria Kotroni, Aggeliki Siamidi, Marilena Vlachou, Vassilios Roussis, Ioannis Sfiniadakis, Efstathia Ioannou, Eleftheria Simirioti, Michail Rallis, Stefanos Kikionis, Vangelis Karalis, Andreas Vitsos
Jazyk: angličtina
Rok vydání: 2019
Předmět:
skin
mice
Erythema
medicine.drug_class
Pinus halepensis bark extract
02 engineering and technology
Pharmacology
lcsh:Technology
electrospun nanofibrous patches
Article
Anti-inflammatory
03 medical and health sciences
0302 clinical medicine
In vivo
medicine
General Materials Science
lcsh:Microscopy
UV-induced inflammation
anti-inflammatory activity
lcsh:QC120-168.85
Transepidermal water loss
lcsh:QH201-278.5
integumentary system
Chemistry
lcsh:T
Dermatological diseases
021001 nanoscience & nanotechnology
3. Good health
Hairless
Pinus
lcsh:TA1-2040
030220 oncology & carcinogenesis
visual_art
visual_art.visual_art_medium
Bark
lcsh:Descriptive and experimental mechanics
lcsh:Electrical engineering. Electronics. Nuclear engineering
medicine.symptom
0210 nano-technology
lcsh:Engineering (General). Civil engineering (General)
lcsh:TK1-9971
Zdroj: Materials
Volume 12
Issue 16
Materials, Vol 12, Iss 16, p 2596 (2019)
ISSN: 1996-1944
DOI: 10.3390/ma12162596
Popis: Skin inflammation is the most common symptom in dermatological diseases. It is usually treated by topically applied products, such as creams, gels and lotions. Skin dressings offer a promising alternative as they are endowed with more controlled administration conditions. In this study, the anti-inflammatory activity of electrospun alginate micro/nanofibrous dressings loaded with the aqueous extract of Pinus halepensis bark (PHBE) was evaluated in vivo in mice. The upper back skin of SKH-1 female hairless mice was exposed to a single dose of ultraviolet radiation (3 MEDs) and the inflamed area was treated daily by the direct application of a nanofibrous patch. The condition of the skin was evaluated primarily on the basis of clinical observation, photo-documentation and histopathological assessment, while measurements of the erythema, hydration, transepidermal water loss (TEWL) and sebum production were also taken into account. The results showed that the topical application of alginate micro/nanofibrous dressings loaded with PHBE on UV-inflamed skin significantly attenuated inflammation damage, reducing the healing period. Increase of the loading dose of PHBE resulted in a proportional reduction of the extent, the density and the depth of skin inflammation. With the steadily increasing interest of the skin dressing industry towards nanofibrous matrices, electrospun nonwovens could serve as ideal candidates for the development of multifunctional anti-inflammatory care systems.
Databáze: OpenAIRE
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